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EMAGE:3340

Hhex hematopoietically expressed homeobox ( MGI:96086)
TS10 (Late streak Downs & Davies)
in situ hybridisation

Data Images
EMAGE:3340
Figure 2D of Thomas et al., 1998 [PMID:9389666] .Copyright: This image is from Thomas et al., Development 125:85-94 (1998), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Anterior to the left. black arrowhead, the position of the node (or prospective node); white arrowheads, Hex expression in the blood islands; black arrows: lateral extension of Hhex in anterior endoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:3340Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3340_wholemount_strong_3D_1.wlz
3340_wholemount_moderate_3D_1.wlz
3340_wholemount_possible_3D_1.wlz
3340_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3340_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
yolk sac
detected detected
regionalBy the late streak stage, expression was detected in an increased number of cells in the expanding blood islands and these cells formed a discrete band around the circumference of the visceral yolk sac.
embryo mesoderm
detected detected
single cellExpression was detected in isolated cells in the proximolateral mesoderm immediately distal to the embryonic/extraembryonic junction.
primitive endoderm
detected detected
regionalThe authors state that there is expression in the anterior endoderm extended from just rostral to the node (at the distal tip) to the embryonic/extraembryonic junction.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1196513
Entity Detected:Hhex, hematopoietically expressed homeobox ( MGI:96086)
Sequence:sense strand is shown

>MGI:1196513
CGGAGGCCCTCTGTACCCGTTCCCGCGGACGGTGAACGACTACACGCACGCCCTACTCCGCCACGACCCC
CTGGGCAAGCCCTTGCTCTGGAGCCCCTTCCTCCAGCGACCTCTGCACAAAAGGAAAGGCGGTCAAGTGA
GGTTCTCCAACGACCAGACCGTCGAGCTGGAGAAGAAGTTCGAGACTCAGAAATACCTCTCCCCACCCGA
GAGAAAGCGTCTGGCCAAGATGTTGCAGCTCAGTGAGAGACAGGTCAAAACCTGGTTTCAGAATCGCCGA
GCTAAATGGAGAAGACTGAAACAGGAGAATCCTCAAAGCAACAAAAAGGATGCGTTGGACAGTTTGGACA
CTTCCTGTGAGCAGGGTCAAGACTTGCCCAGTGAACAGAATAAAGGTGCCTCTTTGGATCGTTCGCAGTG
TTCACCCTCCCCAGCCTCTCAGGAAGACCCCGACTCGGAGATCTCAGAGGATTCCGACCAGGAGGTGGAC
ATCGAGGGGGATAAAGGCTACTTTAATGCTGGATGACA
nt 291 - nt 818 of Z21524.1
Notes:The Hhex probe used in this study by Thomas et al., 1998 [PMID:9389666] "spanned positions 291-818 of the Hex cDNA sequence (EMBL accession no. Z21524)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:Late streak Downs & Davies
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Thomas PQ; Brown A; Beddington RS, 1998 [PMID:9389666] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9389666] Thomas PQ, Brown A, Beddington RS 1998 Hex: a homeobox gene revealing peri-implantation asymmetry in the mouse embryo and an early transient marker of endothelial cell precursors. Development (125):85-94
Links:MGI:1306925 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI