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EMAGE:3344

Hhex hematopoietically expressed homeobox ( MGI:96086)
TS09 (6.5 dpc)
in situ hybridisation

Data Images
EMAGE:3344
Figure 2F of Norris et al., 2002 [PMID:12091315] . Copyright: This image is from Norris DP, Development 2002;129():3455-68 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: AVE, anterior visceral endoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:3344Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3344_wholemount_strong_3D_1.wlz
3344_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3344_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive endoderm
detected detected
regionalExpression is anterior.
definitive endoderm
detected detected
The authors state that Hex is expressed in newly formed definitive endoderm (DE) by mid streak stages. Editor's note: this embryo only shows expression in the AVE though.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1196513
Entity Detected:Hhex, hematopoietically expressed homeobox ( MGI:96086)
Sequence:sense strand is shown

>MGI:1196513
CGGAGGCCCTCTGTACCCGTTCCCGCGGACGGTGAACGACTACACGCACGCCCTACTCCGCCACGACCCC
CTGGGCAAGCCCTTGCTCTGGAGCCCCTTCCTCCAGCGACCTCTGCACAAAAGGAAAGGCGGTCAAGTGA
GGTTCTCCAACGACCAGACCGTCGAGCTGGAGAAGAAGTTCGAGACTCAGAAATACCTCTCCCCACCCGA
GAGAAAGCGTCTGGCCAAGATGTTGCAGCTCAGTGAGAGACAGGTCAAAACCTGGTTTCAGAATCGCCGA
GCTAAATGGAGAAGACTGAAACAGGAGAATCCTCAAAGCAACAAAAAGGATGCGTTGGACAGTTTGGACA
CTTCCTGTGAGCAGGGTCAAGACTTGCCCAGTGAACAGAATAAAGGTGCCTCTTTGGATCGTTCGCAGTG
TTCACCCTCCCCAGCCTCTCAGGAAGACCCCGACTCGGAGATCTCAGAGGATTCCGACCAGGAGGTGGAC
ATCGAGGGGGATAAAGGCTACTTTAATGCTGGATGACA
nt 291 - nt 818 of Z21524.1
Notes:The Hhex probe used in this study by Norris et al., 2002 [PMID:12091315] was originally described in Thomas et al., 1998 [PMID:9389666] who describe the probe as spanning "positions 291-818 of the Hex cDNA sequence (EMBL accession no. Z21524)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:6.5 dpc
Theiler Stage:TS09
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Norris DP; Brennan J; Bikoff EK; Robertson EJ, 2002 [PMID:12091315] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:12091315] Norris DP, Brennan J, Bikoff EK, Robertson EJ 2002 The Foxh1-dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. Development (129):3455-68
 [ PMID:9389666] Thomas PQ, Brown A, Beddington RS 1998 Hex: a homeobox gene revealing peri-implantation asymmetry in the mouse embryo and an early transient marker of endothelial cell precursors. Development (125):85-94
Links:MGI:2183486 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI