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EMAGE:3345

Hoxa3 homeobox A3 ( MGI:96175)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3345
Figure 2H of McKay et al., 1994 [PMID:7925021] .Copyright: This image is from McKay et al., Development 120: 2199-2211 (1994), and is displayed with the permission of The Company of Biologists Limited whom owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Longitudinal section of the hindbrain at the stage of approximately 22 somites (dpc 9.5). Autoradiographic silver grains appear red. Image annotations: O, otocyst; Bar: 100 microns.
Expression Pattern Description
Spatial Annotation:
EMAGE:3345EMAGE:3345Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3345_voxel_strong_3D_1.wlz
3345_voxel_possible_3D_1.wlz
3345_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3345_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
rhombomere 01
not detected not detected
homogeneous
rhombomere 02
not detected not detected
homogeneous
rhombomere 03
not detected not detected
homogeneous
rhombomere 04
not detected not detected
homogeneous
rhombomere 05
detected detected
Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
neural tube
detected detected
regionalExpression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
rhombomere 06
detected detected
Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
rhombomere 07
detected detected
Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
rhombomere 08
detected detected
Expression in posterior regions up to the boundary just anterior to the otocysts and coincident with the boundary betwen r4 and r5.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1328144
Entity Detected:Hoxa3, homeobox A3 ( MGI:96175)
Sequence:sense strand is shown

>MGI:1328144
AATTCAGATAGCCTCCTGCACCGGGAGGCACAGGGCTCCGACTTGGGGACTGGCCCCCAGACGAGGTCAG
CATGCCTTTGCCCTTCTGGTCTTTCTTGTACTTCATGCGGCGGTTCTGGAACCAGATCTTGATCTGGCGC
TCGGTGAGGTTCAGCAGGTTGGCCATCTCCACCCGGCGCGGCCGGCATAGGTAGCGGTTGAAGTGGAACT
CCTTCTCCAGCTCTACCAGCTGCGCGCTCGTGTACGCCGTGCGCGCGCGCTTGGACGAGGCCTGCCCAGG
CGGGCTCTTGTCACCAGCGCAGCTCTCCCCTGCCAGGACACAGAGAGGAAGAGCGGTGTCAGGGGCTGCT
ACCGACCCCGCCTAGCTCCCCAGTCCTAGGCCTGCCCCTCCTTTCTTCACCCCACAGAAGCCTCTGTCCA
AGGCCAGAGAGGAGCAGGACTTCAGTGCTGTGGTCCTCTCTTGGTCTGCGGAAACAGCCTGGTTAGCCTG
CCCTTCCTTCCTCTAAGGAGCGAATCAGGGGCTTCTTCAAGGAGAGGACAGAGCTGCAGAGCCTCCTGCT
TGCCTTGCTGGCCTGAACTTCCTCTCTATTTTACTCGGCCAGCTTATTTCCTGGCCCATCAGACAACAA
nt 52100017 - nt 52100645 of NC_000072.4
Notes:The Hoxa3 (Hox-1.5) probe used in this study by McKay et al, 1994 [PMID:7925021] is indicated as that used by Gaunt, 1988 [PMID:2904354] , which in turn is indicated as that described by Gaunt et al., 1986 [PMID:2879244] ie. a HindIII-EcoRI genomic subclone of a larger cosmid clone (the cosmid clone is described by Duboule et al, 1986 [PMID:3019676] ). The probe region is indicated as RNA probe2 in Fig1 of Gaunt et al, 1986. This figure also shows two other restriction sites that are found within the homeobox (BglII and PvuII). Editor's Note: The sequence co-ordinates above were deduced by scanning genomic sequences for EcoRI, PstI, BglII and PvuII cut sites in the region of the Hoxa3 locus.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:(CBA x C3H/He)F1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:McKay IJ; Muchamore I; Krumlauf R; Maden M; Lumsden A; Lewis J, 1994 [PMID:7925021] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7925021] McKay IJ, Muchamore I, Krumlauf R, Maden M, Lumsden A, Lewis J 1994 The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres. Development (120):2199-211
 [ PMID:2452725] Gaunt SJ 1987 Homoeobox gene Hox-1.5 expression in mouse embryos: earliest detection by in situ hybridization is during gastrulation. Development (101):51-60
 [ doi:10.1038/324662a0] [ PMID:2879244] Gaunt SJ, Miller JR, Powell DJ, Duboule D 1986 Homoeobox gene expression in mouse embryos varies with position by the primitive streak stage. Nature (324):662-4
 [ PMID:3019676] Duboule D, Baron A, Mahl P, Galliot B 1986 A new homeo-box is present in overlapping cosmid clones which define the mouse Hox-1 locus. EMBO J (5):1973-80
Links:MGI:1932351 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI