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EMAGE:3355

Atf4 activating transcription factor 4 ( MGI:88096)
TS14 (8.75 dpc)
in situ hybridisation

Data Images
EMAGE:3355
Fig 4e Murphy & Kolsto, 2000 [PMID:11025215] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: oc, optic cup; b1, first branchial arch; h, heart.
Expression Pattern Description
Spatial Annotation:
EMAGE:3355Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3355_wholemount_strong_3D_1.wlz
3355_wholemount_moderate_3D_1.wlz
3355_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3355_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future midbrain
strong strong
prosencephalon
strong strong
1st branchial arch
strong strong
Expression is in a stripe from the hindbrain down into the first branchial arch.
future hindbrain
strong strong
Expression is in a stripe from the hindbrain down into the first branchial arch.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3033629
Entity Detected:Atf4, activating transcription factor 4 ( MGI:88096)
Sequence:sense strand is shown

>MGI:3033629
AGCGGCAACCCCCACCGGCCTAAGCCATGGCGCTCTTCACGAAATCCAGCAGCAGTGTTGCTGTAACGGA
CAAAGATACCTTCGAGTTAAGCACATTCCTGGAATCCAGCAAAGCCCCACAACATGACCGAGATGAGCTT
CCTGAACAGCGAAGTGTTGGCGGGGGACTTGATGACCCCTTCGACCAGTCGGGTTTGGGGGCTGAAGAAA
GCCTAGGTCTCTTAGATGACTATCTGGAGGTGGCCAAGCACTTGAAACCTCATGGGTTCTCCAGCGACAA
GGCGGGCTCCTCGGAATGGCCGGCTATGGATGATGGCTTGGCCAGTGCCTCAGACACCGGCAAGGAGGAT
GCCTTTTCCGGGACAGATTGGATGTTGGAGAAAATGGATCTGAAAGAGTTTGACTTCGATGCTCTGTTTC
GAATGGATGACCTGGAAACCATGCCAGATGAGCTCT
nt 150 - nt 605 of X61507.1
Notes:The ATF4 probe used in this study by Murphy & Kolsto, 2000 [PMID:11025215] is described as "a 450 bp fragment from a PstI site (bp 150; accession no. X61507) to a SacI site (bp 605) in the 5' part of the coding sequence".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:8.75 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Murphy P, Kolsto A, 2000 [PMID:11025215] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9753] [ PMID:10882515] Christoffels VM, Habets PE, Franco D, Campione M, de Jong F, Lamers WH, Bao ZZ, Palmer S, Biben C, Harvey RP, Moorman AF 2000 Chamber formation and morphogenesis in the developing mammalian heart. Dev Biol (223):266-78
Links:MGI:3033632 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI