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EMAGE:3357

Atf4 activating transcription factor 4 ( MGI:88096)
TS12 (8.2 dpc)
in situ hybridisation

Data Images
EMAGE:3357 EMAGE:3357 EMAGE:3357 EMAGE:3357
Fig 4h Murphy & Kolsto, 2000 [PMID:11025215] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000 Fig 4g Murphy & Kolsto, 2000 [PMID:11025215] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000 Fig 4i Murphy & Kolsto, 2000 [PMID:11025215] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000 Fig 4j Murphy & Kolsto, 2000 [PMID:11025215] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
There are transverse sections of an 8.2 dpc embryo. Image annotations: fb, forebrain; hb, hindbrain; nt, neural tube; s, somites; cnc, cranial neural crest; nc, neural crest; nf, neural folds.
Expression Pattern Description
Spatial Annotation:
EMAGE:3357EMAGE:3357EMAGE:3357EMAGE:3357EMAGE:3357Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3357_voxel_moderate_3D_1.wlz
3357_voxel_possible_3D_1.wlz
3357_voxel_strong_3D_1.wlz
3357_voxel_moderate_3D_2.wlz
3357_voxel_possible_3D_2.wlz
3357_voxel_strong_3D_2.wlz
3357_voxel_possible_3D_3.wlz
3357_voxel_strong_3D_3.wlz
3357_voxel_moderate_3D_3.wlz
3357_voxel_possible_3D_4.wlz
3357_voxel_strong_3D_4.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3357_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future hindbrain
strong strong
regionalFig 4h and 4i - Expression is in the lateral neural folds and in the migrating neural crest. Fig 4j (section through the posterior hindbrain) - expression is homogeneous.
future brain
detected detected
trunk somite
detected detected
homogeneoussee Fig 4j
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3033629
Entity Detected:Atf4, activating transcription factor 4 ( MGI:88096)
Sequence:sense strand is shown

>MGI:3033629
AGCGGCAACCCCCACCGGCCTAAGCCATGGCGCTCTTCACGAAATCCAGCAGCAGTGTTGCTGTAACGGA
CAAAGATACCTTCGAGTTAAGCACATTCCTGGAATCCAGCAAAGCCCCACAACATGACCGAGATGAGCTT
CCTGAACAGCGAAGTGTTGGCGGGGGACTTGATGACCCCTTCGACCAGTCGGGTTTGGGGGCTGAAGAAA
GCCTAGGTCTCTTAGATGACTATCTGGAGGTGGCCAAGCACTTGAAACCTCATGGGTTCTCCAGCGACAA
GGCGGGCTCCTCGGAATGGCCGGCTATGGATGATGGCTTGGCCAGTGCCTCAGACACCGGCAAGGAGGAT
GCCTTTTCCGGGACAGATTGGATGTTGGAGAAAATGGATCTGAAAGAGTTTGACTTCGATGCTCTGTTTC
GAATGGATGACCTGGAAACCATGCCAGATGAGCTCT
nt 150 - nt 605 of X61507.1
Notes:The ATF4 probe used in this study by Murphy & Kolsto, 2000 [PMID:11025215] is described as "a 450 bp fragment from a PstI site (bp 150; accession no. X61507) to a SacI site (bp 605) in the 5' part of the coding sequence".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:8.2 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:unknown
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Murphy P, Kolsto A, 2000 [PMID:11025215] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9753] [ PMID:10882515] Christoffels VM, Habets PE, Franco D, Campione M, de Jong F, Lamers WH, Bao ZZ, Palmer S, Biben C, Harvey RP, Moorman AF 2000 Chamber formation and morphogenesis in the developing mammalian heart. Dev Biol (223):266-78
Links:MGI:3033632 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI