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EMAGE:3369

Kdr kinase insert domain protein receptor ( MGI:96683)
TS11 (LHF Downs & Davies)
immunohistochemistry

Data Images
EMAGE:3369
Figure 3B of Downs et al., 1998 [PMID:9778509] . Copyright: This image is from Downs, et al., Development 125:4507-4520. (1998), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: LHF, late headfold stage; B, basal third; M, middle third; D, distal third; al, allantois; am, amnion; ys, yolk sac. The arrow indicates cells containing an internal spot of Flk-1 without concomitant localization in the plasma membrane whereas the arrowhead indicates cells exhibiting Flk-1 staining in the plasma membrane.
Expression Pattern Description
Spatial Annotation:
EMAGE:3369EMAGE:3369Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3369_voxel_strong_3D_1.wlz
3369_voxel_moderate_3D_1.wlz
3369_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3369_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
allantois mesoderm
detected detected
allantois
detected detected
regionalflk-1 [Kdr] was not observed in the mesothelium of the allantois. The percentage of positive core cells was highest in the distal third of the allantoic bud, significantly lower in the basal third and intermediate in the middle third.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1298413
Entity Detected:Kdr, kinase insert domain protein receptor ( MGI:96683)
Notes:The anti-Flk-1(Kdr) antibody used in this study by Downs et al., 1998 [PMID:9778509] is described as a polyclonal antiserum raised in rabbit from Santa Cruz Biotechtechnology. Editor's note: Santa Cruz Biotechnology sell four anti-Flk-1 antisera raised in rabbit. It is unclear which was used by the authors as no catalogue number is specified in the paper.
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Santa Cruz Biotechnology
Specimen
Organism:mouse
Strain:(C57BL/6 x CBA)F1
Age:LHF Downs & Davies
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
General Information
Authors:Downs KM; Gifford S; Blahnik M; Gardner RL, 1998 [PMID:9778509] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9778509] Downs KM, Gifford S, Blahnik M, Gardner RL 1998 Vascularization in the murine allantois occurs by vasculogenesis without accompanying erythropoiesis. Development (125):4507-20
Links:MGI:1298414 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI