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EMAGE:3372

Pax5 paired box gene 5 ( MGI:97489)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3372
Figure 8I of Suda et al., 2001 [PMID:11493561] . Copyright: This image is from Suda et al., Development 128: 2433-2450 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3372Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3372_wholemount_strong_3D_1.wlz
3372_wholemount_moderate_3D_1.wlz
3372_wholemount_possible_3D_1.wlz
3372_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3372_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1345492
Entity Detected:Pax5, paired box gene 5 ( MGI:97489)
Notes:The Pax5 probe used in this study by Suda et al., 2001 [PMID:11493561] is indicated as that originally described by Asano & Gruss, 1992 ( [PMID:null] 1283313) i.e. "5sg8 - a 196 bp putative exon 3 region" (see Figure 1A therein). Exact start and end points are not defined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:undefined
General Information
Authors:Suda Y; Hossain ZM; Kobayashi C; Hatano O; Yoshida M; Matsuo I; Aizawa S, 2001 [PMID:11493561] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493561] Suda Y, Hossain ZM, Kobayashi C, Hatano O, Yoshida M, Matsuo I, Aizawa S 2001 Emx2 directs the development of diencephalon in cooperation with Otx2. Development (128):2433-50
 [ doi:10.1016/0925-4773(92)90023-D] [ PMID:1283313] Asano M, Gruss P 1992 Pax-5 is expressed at the midbrain-hindbrain boundary during mouse development. Mech Dev (39):29-39
Links:MGI:2148286 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI