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EMAGE:3376

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:3376
Figure 6A of Faber et al., 2001 [PMID:11714669] . Copyright: This image is from Faber SC, Development 2001;128():4425-4438 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: The lens placode is indicated by the arrowheads. Both the green and yellow colours show Pax6 immunoreactivity, but the yellow shows the peak signal intensity. OV, optic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:3376EMAGE:3376Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3376_voxel_strong_3D_1.wlz
3376_voxel_moderate_3D_1.wlz
3376_voxel_weak_3D_1.wlz
3376_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3376_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
lens placode
strong strong
optic cup intraretinal space
detected detected
Expression is detected in the presumptive retina.
optic cup outer layer
detected detected
Expression is detected in the presumptive retina.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2157328
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Antigen:sense strand is shown

>MGI:2157328
QVPGSEPDMSQYWPRLQ
aa 420 - aa 436 of NP_038655.1
Notes:The anti-Pax6 antibody used in this study by Faber et al., 2001 [PMID:11714669] is described as an "affinity purified rabbit polyclonal available from Covance (cat#PRB-278P)". Editor's note: Information from the Covance website (http://www.crpinc.com/) states "This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a peptide affinity column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes".
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Covance
Catalogue Number:PRB-278P
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Visualisation method:fluorescence
General Information
Authors:Faber SC; Dimanlig P; Makarenkova HP; Shirke S; Ko K; Lang RA, 2001 [PMID:11714669] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11714669] Faber SC, Dimanlig P, Makarenkova HP, Shirke S, Ko K, Lang RA 2001 Fgf receptor signaling plays a role in lens induction. Development (128):4425-38
 [ PMID:8756438] Davis JA, Reed RR 1996 Role of Olf-1 and Pax-6 transcription factors in neurodevelopment. J Neurosci (16):5082-94
Links:MGI:2157343 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI