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EMAGE:3378

Pax6 paired box gene 6 ( MGI:97490)
TS16 (27 Somite no.)
immunohistochemistry

Data Images
EMAGE:3378 EMAGE:3378
Figure 1D of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 1F of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Pax6 expression in green, Nkx2.1 expression in red, Gsh2 expression in blue. Arrows, gap between Pax6 and Nkx2.1 expression. Ctx, cerebral cortex; LGE, presumptive future lateral ganglionic eminence; MGE, presumptive future medial ganglionic eminence. Only the expression of Pax6 has been denoted in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:3378EMAGE:3378Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3378_voxel_strong_3D_1.wlz
3378_voxel_moderate_3D_1.wlz
3378_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3378_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalThere is a slight gap between Pax6 and Nkx2.1 expression.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2157328
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Antigen:sense strand is shown

>MGI:2157328
QVPGSEPDMSQYWPRLQ
aa 420 - aa 436 of NP_038655.1
Notes:The anti-PAX6 antibody used in this study by Corbin et al, 2003 [PMID:12930780] is described as "rabbit anti-Pax6 (1:1000, Covance, CA, USA)". Editor's note: Covance (http://www.crpinc.com/) make one anti-PAX6 antisera (PRB-278P). Info from the Covance online catalogue states "This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a peptide affinity column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes".
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Covance
Catalogue Number:PRB-278P
Specimen
Organism:mouse
Age:27 Somite no.
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Visualisation method:fluorescence
General Information
Authors:Corbin JG; Rutlin M; Gaiano N; Fishell G, 2003 [PMID:12930780] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00717] [ PMID:12930780] Corbin JG, Rutlin M, Gaiano N, Fishell G 2003 Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development (130):4895-906
Links:MGI:2676225 same experiment
 EMAGE:3433 same section & embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI