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EMAGE:3381

Pecam1 platelet/endothelial cell adhesion molecule 1 ( MGI:97537)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:3381 EMAGE:3381
Figure 5C of Lin et al., 1998 [PMID:9778514] . Copyright: This image is from Lin et al., Development 125: 4565-4574 (1998), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 5A of Lin et al., 1998 [PMID:9778514] . Copyright: This image is from Lin et al., Development 125: 4565-4574 (1998), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
C is a section taken from the whole embryo in A. Image annotations: da, dorsal aorta; lv, left ventricle; nt, neural tube; rv, right ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:3381EMAGE:3381EMAGE:3381Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3381_wholemount_strong_3D_2.wlz
3381_wholemount_moderate_3D_2.wlz
3381_wholemount_possible_3D_1.wlz
3381_voxel_strong_3D_1.wlz
3381_voxel_moderate_3D_1.wlz
3381_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3381_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression is seen in endothelial cell network throughout the embryo.
heart
detected detected
regionalExpression is seen in the aortic arch arteries and endocardium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1328239
Entity Detected:Pecam1, platelet/endothelial cell adhesion molecule 1 ( MGI:97537)
Notes:The anti-PECAM1 antibody used in this study by Lin et al., 1998 [PMID:9778514] was an "anti-mouse PECAM monoclonal antibody MEC13.3 (Pharmingen)." Editor's Note: Pharmingen sell 8 various anti-PECAM-1 antisera derived from clone MEC13.3.
Antibody Type:monoclonal
Raised In:rat
Supplier:Pharmingen
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Secondary Antibody:goat anti-rat
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Lin Q; Lu J; Yanagisawa H; Webb R; Lyons GE; Richardson JA; Olson EN, 1998 [PMID:9778514] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9778514] Lin Q, Lu J, Yanagisawa H, Webb R, Lyons GE, Richardson JA, Olson EN 1998 Requirement of the MADS-box transcription factor MEF2C for vascular development. Development (125):4565-74
Links:MGI:1328240 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI