EMAGE mouse gene expression spatial database

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EMAGE:3387

T brachyury ( MGI:98472)
TS11 (7.5 dpc)
in situ hybridisation

Data Images


Figure 1C of Herrmann BG, 1991 [PMID:1821859] . Copyright: This image is from Herrmann, Development 113:913-917 (1991), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: ps, primitive streak; hp, head process.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3387_wholemount_strong_3D_1.wlz

3387_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3387_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

notochordal process

detected

primitive streak

detected

embryo mesoderm

not detected

homogeneous

Expression is down-regulated to undetectable levels in mesoderm cells migrating away from the streak.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2449092
Entity Detected:	T, brachyury ( MGI:98472)
Sequence:	sense strand is shown >MGI:2449092 GGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGG AGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAA GAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAG GGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGC TAACTAACGAGATGATTGTGACCAAGAACGGCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGG CCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATAT GTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAG ACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAA GCTCAATGGAGGGGGACAGATCATGTTAAACTCCTTGCATAAGTATGAACCTCGGATTCACATCGTGAGA GTTGGGGGCCCGCAACGCATGATCACCAGCCACTGCTTTCCCGAGACCCAGTTCATAGCTGTGACTGCCT ACCAGAATGAGGAGATTACAGCCCTTAAAATTAAATACAACCCATTTGCTAAAGCCTTCCTTGATGCCAA AGAAAGAAACGACCACAAAGATGTAATGGAGGAACCGGGGGACTGCCAGCAGCCGGGGTATTCCCAATGG GGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGC TCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCC CAGCCCCTATGCTCATCGGAACAGCTCTCCAACCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTG CAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACG CCAGCCCACCTACTGGCTCTAGCCAGTATCCCAGTCTCTGGTCTGTGAGCAATGGTACCATCACCCCAGG CTCCCAGACAGCTGGGGTGTCCAACGGGCTGGGAGCTCAGTTCTTTCGAGGCTCCCCTGCACATTACACA CCACTGACGCACACGGTCTCAGCTGCCACGTCCTCGTCTTCTGGTTCTCCGATGTATGAAGGGGCTGCTA CAGTCACAGACATTTCTGACAGCCAGTATGACACGGCCCAAAGCCTCCTCATAGCCTCGTGGACACCTGT GTCACCCCCATCTATGTGAATTGAACTTTCCTCCATGTGCTGAGACTTGTAACAACCGGTGTCAACTGGA TCTTCTAGGCTCAAAGTGGCAGGCTCTTGGGACAAGGGAAAAATAAATAAATAAAAGCTAGATACTAACA ACTCCATTTTCAAATAAGAGCAATAATACATGTCCTATAATCATGTTCTACAGCCTCTTGTTTGATACCT ACAGTAGTGATATGTGTCCTACATTATGAAGCCAAGGACAGAGAGACGGCTGTGGTCCAGTTTTTTGTGA CTGGCAGTTAATCAGAGTCCTTTGCTAGGTAGGGTCCTATATCTTGTGTTTCTCTACAACATATATGTGA CTTTGAAATCCTGG
	nt 1 - nt 1764 of X51683.1
Notes:	The T probe used in this study by Herrmann, 1991 [PMID:1821859] is described as "the sequence from base 1 to base 1764 of the cDNA for the T gene, pme75 (Herrmann et al., 1990 [PMID:2154694] )."
Chemistry:	RNA
Strand:	antisense
Label:	biotin

Specimen

Organism:	mouse
Age:	7.5 dpc
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	B-gal +XGal

General Information

Authors:	Herrmann BG, 1991 [PMID:1821859] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:1821859] Herrmann BG 1991 Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development (113):913-7
	[ doi:10.1038/343617a0] [ PMID:2154694] Herrmann BG, Labeit S, Poustka A, King TR, Lehrach H 1990 Cloning of the T gene required in mesoderm formation in the mouse. Nature (343):617-22
Links:	MGI:1345707 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source