Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:339

Wnt3a wingless-related MMTV integration site 3A ( MGI:98956)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:339 EMAGE:339
Figure 1G of Sporle et al., 1996 [PMID:8565855] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1H of Sporle et al., 1996 [PMID:8565855] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a double in situ for Wnt3a and Myf5. Only the Wnt3a expression has been text and spatially mapped in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:339Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
339_wholemount_strong_3D_1.wlz
339_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:339_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
regionalExpression is in the dorsal-most area of the future spinal cord.
neural tube roof plate
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334562
Entity Detected:Wnt3a, wingless-related MMTV integration site 3A ( MGI:98956)
Sequence:sense strand is shown

>MGI:1334562
GAATTCCAATTTGGGCCGGAAGTCCTACCTCAATGGCTTGGACTCCTCTCTTGACCCGACAGGGCTCAAA
TGGAGACAGGTAAGCTACTCCCTCAACTAGGTGGGGTTCGTGCGGATGGGTGGGAGGGGAGAGATTAGGG
TCCCTCCTCCCAGAGGCACTGCTCTATCTAGATACATGAGAGGGTGCTTCAGGGTGGGCCCTATTTGGGC
TTGAGGATCCCGTGGGGGCGGGGCTTCACCCCGACTGGGTGGAACTTTTGGAGACCCCCTTCCACTGGGG
CAAGGCTTCACTGAAGACTCATGGGATGGAGCTCCACGGAAGGAGGAGTTCCTGAGCGAGCCTGGGCTCT
GAGCAGGCCATCCAGCTCCCATCTGGCCCCTTTCCAGTCCTGGTGTAAGGTTCAACCTGCAAGCCTCATC
TGCGCAGAGCAGGATCTCCTGGCAGAATGAGGCATGGAGAAGAACTCAGGGGTGATACCAAGACCTAACA
AACCCCGTGCCTGGGTACCTCTTTTAAAGCTCTGCACCCCTTCTTCAAGGGCTTTCCTAGTCTCCTTGGC
AGAGCTTTCCTGAGGAAGATTTGCAGTCCCCCAGAGTTCAAGTGAACACCCATAGAACAGAACAGACTCT
ATCCTGAGTAGAGAGGGTTCTCTAGGAATCTCTATGGGGACTGCTAGGAAGGATCCTGGGCATGACAGCC
TCGTATGATAGCCTGCATCCGCTCTGACACTTAATACTCA
nt 1452 - nt 2191 of X56842.1
Notes:The Wnt3a probe used in this study by Sporle et al., 1996 [PMID:8565855] was previously described by Roelink and Nusse, 1991 [PMID:2001840] as a "750-bp EcoRI-BglII fragment","derived from noncoding and nonconserved cDNA fragments".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Sporle et al., 1996 [PMID:8565855] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8565855] Sporle R, Gunther T, Struwe M, Schughart K 1996 Severe defects in the formation of epaxial musculature in open brain (opb) mutant mouse embryos. Development (122):79-86
 [ doi:10.1101/gad.5.3.381] [ PMID:2001840] Roelink H, Nusse R 1991 Expression of two members of the Wnt family during mouse development--restricted temporal and spatial patterns in the developing neural tube. Genes Dev (5):381-8
Links:MGI:1336670 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI