EMAGE mouse gene expression spatial database

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EMAGE:3401

Dlx5 distal-less homeobox 5 ( MGI:101926)
TS14 (9 dpc)
in situ hybridisation

Data Images


Figure 1G of Depew et al., 1999 [PMID:10433912] . Copyright: This image is from Depew MJ, Development 1999;126():3831-46, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 1F of Depew et al., 1999 [PMID:10433912] . Copyright: This image is from Depew MJ, Development 1999;126():3831-46, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 1H of Depew et al., 1999 [PMID:10433912] . Copyright: This image is from Depew MJ, Development 1999;126():3831-46, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:
Find spatially similar expression patterns:

Notes:

Note: The planes of the sections shown in G and H are shown in F. Abbreviations: aCE=anterior cephalic actoderm, BA1=first branchial arch, dNT= dorsal neural tube, OPt= otic pit, OV= otic vesicle, pN= posterior neuropore. The wholemount in 1F and the section in 1G have been spatially annotated in this EMAGE entry.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

wholemount mapping

View mapped 3D expression image

Download individual expression domains:

3401_wholemount_strong_3D_2.wlz

3401_wholemount_moderate_3D_2.wlz

3401_wholemount_notDetected_3D_2.wlz

3401_voxel_strong_3D_1.wlz

3401_voxel_moderate_3D_1.wlz

3401_voxel_possible_3D_1.wlz

3401_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3401_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

1st branchial arch ectoderm

detected

regional

Expression is distal. (Figs 1F&G)

1st branchial arch mesenchyme

detected

regional

Expression is distal. (Figs 1F&G)

caudal neuropore

detected

see Fig 1F

neural tube roof plate

detected

regional

1G shows that there is no expression anteriorly, but in other sections at more caudal regions levels there is expression (e.g. 1H).

otic pit

detected

see Fig 1F

embryo ectoderm

detected

Expression is in the anterior cephalic ectoderm. See Fig 1F

otic pit epithelium

detected

graded

Expression is strongest at the dorsal margin.(Fig 1G)

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1339070
Entity Detected:	Dlx5, distal-less homeobox 5 ( MGI:101926)
Notes:	The Dlx5 probe used in this study by Depew et al., 1999 [PMID:10433912] was originally described in Qiu et al., 1997 [PMID:9187081] as "a 1-kb cDNA containing its homeobox and 3' sequences (Liu and Rubenstein, unpublished)." Editors note: exact start and end points of the probe sequence are undefined.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	C57BL/6J
Age:	9 dpc
Theiler Stage:	TS14
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Depew MJ; Liu JK; Long JE; Presley R; Meneses JJ; Pedersen RA; Rubenstein JL, 1999 [PMID:10433912] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:10433912] Depew MJ, Liu JK, Long JE, Presley R, Meneses JJ, Pedersen RA, Rubenstein JL 1999 Dlx5 regulates regional development of the branchial arches and sensory capsules. Development (126):3831-46
	[ doi:10.1006/dbio.1997.8556] [ PMID:9187081] Qiu M, Bulfone A, Ghattas I, Meneses JJ, Christensen L, Sharpe PT, Presley R, Pedersen RA, Rubenstein JL 1997 Role of the Dlx homeobox genes in proximodistal patterning of the branchial arches: mutations of Dlx-1, Dlx-2, and Dlx-1 and -2 alter morphogenesis of proximal skeletal and soft tissue structures derived from the first and second arches. Dev Biol (185):165-84
Links:	MGI:1342230 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source