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EMAGE:3404

Pou3f3 POU domain, class 3, transcription factor 3 ( MGI:102564)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3404
Figure 2F of Bouchard et al., 2005 [PMID:15872005] . Copyright: This image is from Bouchard M, Development 2005 Jun;132(11):2633-43 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: E9.5 embryo. Arrowhead indicates the position of the mid-hindbrain boundary (mhb). Abbreviations: ms, mesonephros; sc, spinal cord.
Expression Pattern Description
Spatial Annotation:
EMAGE:3404Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3404_wholemount_strong_3D_1.wlz
3404_wholemount_moderate_3D_1.wlz
3404_wholemount_possible_3D_1.wlz
3404_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3404_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
prosencephalon
detected detected
future hindbrain
detected detected
future midbrain
detected detected
homogeneousExpression was detected throughout the entire midbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3583711
Entity Detected:Pou3f3, POU domain, class 3, transcription factor 3 ( MGI:102564)
Sequence:sense strand is shown

>MGI:3583711
TGGCGTCGTCGGTGGAGAACACCTGCCTCGGAGATAAGAGAGAGACACCCCCCCCCTCTTTCTTCCACTT
CTTCCAAAAGGGCTGCCTAAAAGATCCATGGAAACTTTCTTTATGGGGAGCGACCTGAAAAGAAAGACGC
ACCAGGTTTCCTGCAGGTCTGACTCTGCTCTTGGACAGTGTTTTTCTTGGCCATATTCAAGTCTTTGGGG
AACAAATAAATCAACTAAGAAATGGGAAGAAGGGAGAAACAGAGTCTGGGGGGGAAGATTCGCCGCTTCT
GAAGTTCACAGACTGAACAAGAAAGCCAAAAGAAAGCATCCTCCGCAGTAAACTTGGAAATAAAAGCATC
GCGAAAGAGCCAAGGCTGGGTGAGGCTGAAGGGTAGGTACGTCCAGCCGCAATCCACTAGCGGAGAGGGC
ACAAAAGCGGGGCCCGGGCCCAGCCTCTGAGACCGCCCACAAGGGCCAGGCCTCCACCACCAAACCCGGG
CTTTGCTGACTGCCGCTCGGGGTGGACCATGCCTAAAGATTCCGCCGCTAATATTTTTTTTATTAACATT
TTTTATTTTTTATTTCTGGACTGACTCCGATAAAAGGGTGTGGAAAGACAGCACCAACCGCGCACTTCCC
TTGACTTCTGCCCT
nt 2012 - nt 2655 of NM_008900.2
Notes:The Pou3f3 probe used in this study by Bouchard et al., 2005 [PMID:15872005] was a "mouse Brn1 [Pou3f3] probe (643 bp) [that] was cloned into the pGEM-Teasy plasmid (Promega) following RT-PCR from E10.5 head cDNA using the primers 5'-GGGCAGAAGTCAAGGGAAGTG-3' and 5'-TGGCGTCGTCGGTGGAGAACA-3'". Editors Note: co-ordinates against NM_008900.2 were deduced using this information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Bouchard M; Grote D; Craven SE; Sun Q; Steinlein P; Busslinger M, 2005 [PMID:15872005] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01833] [ PMID:15872005] Bouchard M, Grote D, Craven SE, Sun Q, Steinlein P, Busslinger M 2005 Identification of Pax2-regulated genes by expression profiling of the mid-hindbrain organizer region. Development (132):2633-43
Links:MGI:3586674 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI