Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:341

Wnt3a wingless-related MMTV integration site 3A ( MGI:98956)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:341
Figure 4A of Gunther et al., 1994 [PMID:7720556] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: CC - central canal; DRG - dorsal root ganglia; FP - floor plate; RP - roof plate; VH - ventral horn.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
spinal cord
detected detected
regionalExpression in the dorsalmost region of the developing spinal cord including the roof plate.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334562
Entity Detected:Wnt3a, wingless-related MMTV integration site 3A ( MGI:98956)
Sequence:sense strand is shown

>MGI:1334562
GAATTCCAATTTGGGCCGGAAGTCCTACCTCAATGGCTTGGACTCCTCTCTTGACCCGACAGGGCTCAAA
TGGAGACAGGTAAGCTACTCCCTCAACTAGGTGGGGTTCGTGCGGATGGGTGGGAGGGGAGAGATTAGGG
TCCCTCCTCCCAGAGGCACTGCTCTATCTAGATACATGAGAGGGTGCTTCAGGGTGGGCCCTATTTGGGC
TTGAGGATCCCGTGGGGGCGGGGCTTCACCCCGACTGGGTGGAACTTTTGGAGACCCCCTTCCACTGGGG
CAAGGCTTCACTGAAGACTCATGGGATGGAGCTCCACGGAAGGAGGAGTTCCTGAGCGAGCCTGGGCTCT
GAGCAGGCCATCCAGCTCCCATCTGGCCCCTTTCCAGTCCTGGTGTAAGGTTCAACCTGCAAGCCTCATC
TGCGCAGAGCAGGATCTCCTGGCAGAATGAGGCATGGAGAAGAACTCAGGGGTGATACCAAGACCTAACA
AACCCCGTGCCTGGGTACCTCTTTTAAAGCTCTGCACCCCTTCTTCAAGGGCTTTCCTAGTCTCCTTGGC
AGAGCTTTCCTGAGGAAGATTTGCAGTCCCCCAGAGTTCAAGTGAACACCCATAGAACAGAACAGACTCT
ATCCTGAGTAGAGAGGGTTCTCTAGGAATCTCTATGGGGACTGCTAGGAAGGATCCTGGGCATGACAGCC
TCGTATGATAGCCTGCATCCGCTCTGACACTTAATACTCA
nt 1452 - nt 2191 of X56842.1
Notes:The Wnt3a probe used in this study by Gunther et al., 1994 [PMID:7720556] was previously described by Roelink and Nusse, 1991 [PMID:2001840] as a "750-bp EcoRI-BglII fragment","derived from noncoding and nonconserved cDNA fragments".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
General Information
Authors:Gunther et al., 1994 [PMID:7720556] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7720556] Gunther T, Struwe M, Aguzzi A, Schughart K 1994 Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord. Development (120):3119-30
 [ doi:10.1101/gad.5.3.381] [ PMID:2001840] Roelink H, Nusse R 1991 Expression of two members of the Wnt family during mouse development--restricted temporal and spatial patterns in the developing neural tube. Genes Dev (5):381-8
Links:MGI:1933414 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI