Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3431

Nkx2-1 NK2 homeobox 1 ( MGI:108067)
TS16 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:3431
Figure 1B of Corbin et al., 2000 [PMID:11060228] . Copyright: This image is from Corbin et al., Development 127:5007-20 (2000), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: In situ analysis of Gsh2 protein (green) and Titf1 protein (red) expression in coronal sections. ***Only the Titf1 expression domain is described in this EMAGE Entry***
Expression Pattern Description
Spatial Annotation:
EMAGE:3431EMAGE:3431Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3431_voxel_strong_3D_1.wlz
3431_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3431_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalExpression in ventrolateral telencephalon
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2153074
Entity Detected:Nkx2-1, NK2 homeobox 1 ( MGI:108067)
Notes:The anti-Titf1 (Nkx2.1/Ttf1) antibody used in this study by Corbin et al., 2000 [PMID:11060228] was a "mouse anti-NKX2.1 (1:2000, gift of J. Whitsett)". Editors note: Literature searching identifies the anti-Titf (Ttf1) antisera. Its production method is outlined in Holzinger etal., 1996 [PMID:9064286] .
Antibody Type:monoclonal
Raised In:mouse
Supplier:J. Whitsett
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Labelled with:Cy3
Visualisation method:fluorescence
General Information
Authors:Corbin JG; Gaiano N; Machold RP; Langston A; Fishell G, 2000 [PMID:11060228] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11060228] Corbin JG, Gaiano N, Machold RP, Langston A, Fishell G 2000 The Gsh2 homeodomain gene controls multiple aspects of telencephalic development. Development (127):5007-20
 [ doi:10.1089/hyb.1996.15.49] [ PMID:9064286] Holzinger A, Dingle S, Bejarano PA, Miller MA, Weaver TE, DiLauro R, Whitsett JA 1996 Monoclonal antibody to thyroid transcription factor-1: production, characterization, and usefulness in tumor diagnosis. Hybridoma (15):49-53
Links:MGI:2153160 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI