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EMAGE:3442

Htr2b 5-hydroxytryptamine (serotonin) receptor 2B ( MGI:109323)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:3442
Figure 3H of Choi et al., 1997 [PMID:9165122] . Copyright: This image is from Choi, et al., Development 124:1745-1755 (1997), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: b, forebrain region; h, heart; op, optic vesicle; pa1, first pharyngeal arch; *, staining of caudal region sometimes revealed in controls (may be non-specific).
Expression Pattern Description
Spatial Annotation:
EMAGE:3442Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3442_wholemount_strong_3D_1.wlz
3442_wholemount_moderate_3D_1.wlz
3442_wholemount_weak_3D_1.wlz
3442_wholemount_possible_3D_1.wlz
3442_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3442_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
trunk somite
detected detected
heart
detected detected
neural tube
detected detected
otocyst
detected detected
optic vesicle
detected detected
tail neural tube
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1332680
Entity Detected:Htr2b, 5-hydroxytryptamine (serotonin) receptor 2B ( MGI:109323)
Antigen:sense strand is shown

>MGI:1332680
STIQSSSIILLDTLLTENDGDKAEEQVSYI
aa 450 - aa 479 of NP_032337.2
Notes:The anti-Htr2b antibody used in this study by Choi et al., 1997 [PMID:9165122] was originally described in Choi & Maroteaux, 1996 [PMID:8706927] as being raised against the "C-terminal part of mouse 5-HT2B receptor. The sequence has the following 31 amino acid sequence: CSTIQSSSIILLDTLLTENDGDKAEEQVSYI. For immunisation, the peptide (2 mg) was coupled through cystein to ovalbumin (10 mg) as a carrier protein, using m-maleimidobenzoyl-N- hyroxysuccinimde (MBS) as the linking agent." The antibody was subsequently affinity purified.
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Choi DS; Ward SJ; Messaddeq N; Launay JM; Maroteaux L, 1997 [PMID:9165122] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9165122] Choi DS, Ward SJ, Messaddeq N, Launay JM, Maroteaux L 1997 5-HT2B receptor-mediated serotonin morphogenetic functions in mouse cranial neural crest and myocardiac cells. Development (124):1745-55
 [ doi:10.1016/0014-5793(96)00695-3] [ PMID:8706927] Choi DS, Maroteaux L 1996 Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain. FEBS Lett (391):45-51
Links:MGI:1332681 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI