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EMAGE:3443

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3443 EMAGE:3443
Figure 7-Otlx2-left of Daubas et al., 2000 [PMID:10603349] . Copyright: This image is from Daubas P, Development 2000;127():319-331, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 7-Otlx2-right of Daubas et al., 2000 [PMID:10603349] . Copyright: This image is from Daubas P, Development 2000;127():319-331, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: mv, mesencephalic vesicle. The arrows point to expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:3443EMAGE:3443Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3443_voxel_strong_3D_1.wlz
3443_voxel_possible_3D_1.wlz
3443_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3443_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalExpression is detected in the ventral mesencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1194845
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 (Otlx2) probe used in this study by Daubas et al., 2000 [PMID:10603349] was originally described in Mucchielli et al., 1996 [PMID:9026314] . Editors note: The template clone is simply described as a pBluescript KS II clone by Mucchielli et al and no information regarding the exact insert sequence is given.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Myf5+/Myf5tm1Pas
Preparation:section
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Daubas P; Tajbakhsh S; Hadchouel J; Primig M; Buckingham M, 2000 [PMID:10603349] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10603349] Daubas P, Tajbakhsh S, Hadchouel J, Primig M, Buckingham M 2000 Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development (127):319-31
 [ doi:10.1006/mcne.1996.0062] [ PMID:9026314] Muccielli ML, Martinez S, Pattyn A, Goridis C, Brunet JF 1996 Otlx2, an Otx-related homeobox gene expressed in the pituitary gland and in a restricted pattern in the forebrain. Mol Cell Neurosci (8):258-71
Links:MGI:1352727 same experiment
 MGI:1857973 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI