Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:345

Wnt3 wingless-related MMTV integration site 3 ( MGI:98955)
TS09 (6.5 dpc)
in situ hybridisation

Data Images
EMAGE:345
Figure 2D (top) of Norris et al., 2002 [PMID:12091315] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image Notes: anterior is towards the left of the image. The embryo is also described as primitive streak stage.
Expression Pattern Description
Spatial Annotation:
EMAGE:345Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
345_wholemount_strong_3D_1.wlz
345_wholemount_moderate_3D_1.wlz
345_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:345_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo ectoderm
detected detected
regionalExpression is confined to the posterior proximal epiblast.
primitive endoderm
detected detected
regionalExpression is in the area overlying the posterior proximal epiblast.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2182553
Entity Detected:Wnt3, wingless-related MMTV integration site 3 ( MGI:98955)
Notes:The Wnt3 probe used in this study by Norris et al., 2002 [PMID:12091315] is indicated as being previously described by Liu et al., 1999 [PMID:10431240] ie. as a "0.8-kb cDNA fragment containing exons 3-5 (Roelink et al., 1990 [PMID:2162045] ) ". Roelink et al. refer to two Wnt3 cDNA clones, neither of which are 0.8kb.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:ICR
Age:6.5 dpc
Theiler Stage:TS09
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Norris et al., 2002 [PMID:12091315] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:12091315] Norris DP, Brennan J, Bikoff EK, Robertson EJ 2002 The Foxh1-dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. Development (129):3455-68
 [ doi:10.1038/11932] [ PMID:10431240] Liu P, Wakamiya M, Shea MJ, Albrecht U, Behringer RR, Bradley A 1999 Requirement for Wnt3 in vertebrate axis formation. Nat Genet (22):361-5
 [ doi:10.1073/pnas.87.12.4519] [ PMID:2162045] Roelink H, Wagenaar E, Lopes da Silva S, Nusse R 1990 Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain. Proc Natl Acad Sci U S A (87):4519-23
Links:MGI:2183483 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI