EMAGE mouse gene expression spatial database

EMAGE:3454

Fgf10 fibroblast growth factor 10 ( MGI:1099809)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Figure 3A of Rallis et al., 2003 [PMID:12736217] . Copyright: This image is from Rallis et al., Development 130:2741-2751 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 3C of Rallis et al., 2003 [PMID:12736217] . Copyright: This image is from Rallis et al., Development 130:2741-2751 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
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Notes:

Note: Black arrow indicates forelimb bud, * indicates expression in forelimb bud.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb bud

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1194639
Entity Detected:	Fgf10, fibroblast growth factor 10 ( MGI:1099809)
Sequence:	sense strand is shown >MGI:1194639 GGATACTGACACATTGTGCCTCAGCCTTTCCCCACCTGCCGGGCTGCTGTTGCTGCTTCTTGTTGCTCTT TTTGGTGTCTTCGTTCCCTGTCACCTGCCAAGCTCTTGGTCAGGACATGGTGTCACAGGAGGCCACCAAC TGCTCTTCTTCCTCCTCGTCCTTCTCCTCTCCTTCCAGTGCGGGAAGGCATGTGCGGAGCTACAATCACC TCCAAGGAGATGTCCGCTGGAGAAGGCTGTTCTCCTTCACCAAGTACTTTCTCACGATTGAGAAGAACGG CAAGGTCAGCGGGACCAAGAATGAAGACTGTCCGTACAGTGTCCTGGAGATAACATCAGTGGAAATCGGA GTTGTTGCCGTCAAAGCCATCAACAGCAACTATTACTTAGCCATGAACAAGAAGGGGAAACTCTATGGCT CAAAAGAGTTTAACAACGACTGTAAGCTGAAAGAGAGAATAGAGGAAAATGGATACAACACCTATGCATC TTTTAACTGGCAGCACAATGGCAGGCAAATGTATGTGGCATTGAATGGAAAAGGAGCTCCCAGGAGAGGA CAAAAAACA
	nt 361 - nt 929 of NM_008002.3
Notes:	The Fgf10 probe used in this study by Rallis et al., 2003 [PMID:12736217] is indicated as that originally used by Bellusci et al., 1997 [PMID:9428423] i.e. a "584bp fragment" of "murine Fgf10", "cloned by RT-PCR from E11.5 mouse lung cDNA. The forward primer was +11 to +34 in the rat sequence (5' GGATACTGACACATTGTGCCTCAG 3') and the reverse primer was +574 to +597 (5' TGTTTTTTGTCCTCTCCTGGGAG 3') (Yamasaki et al, 1996 [PMID:8663172] )". Editor's note: When compared against the mouse Fgf10 cDNA RefSeq NM_008002.3, these primers identify a 568 nt fragment as opposed to a 584nt fragment as described by the authors.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Rallis C; Bruneau BG; Del Buono J; Seidman CE; Seidman JG; Nissim S; Tabin CJ; Logan MP, 2003 [PMID:12736217] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.00473] [ PMID:12736217] Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP 2003 Tbx5 is required for forelimb bud formation and continued outgrowth. Development (130):2741-51
	[ PMID:9428423] Bellusci S, Grindley J, Emoto H, Itoh N, Hogan BL 1997 Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development (124):4867-78
	[ doi:10.1074/jbc.271.27.15918] [ PMID:8663172] Yamasaki M, Miyake A, Tagashira S, Itoh N 1996 Structure and expression of the rat mRNA encoding a novel member of the fibroblast growth factor family. J Biol Chem (271):15918-21
Links:	MGI:2677745 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source