Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:346

Wnt2b wingless related MMTV integration site 2b ( MGI:1261834)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:346 EMAGE:346
Figure 4H of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 4C of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: OC - optic cup; OV - otic vesicle. C is a dorsal view of the forebrain at the same stage as the embryo in H.
Expression Pattern Description
Spatial Annotation:
EMAGE:346Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
346_wholemount_strong_3D_1.wlz
346_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:346_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
not detected not detected
No expression in the medial wall of the telencephalon.
diencephalon
detected detected
regionalExpression at the dorsal midline of the diencephalon and midbrain.
midbrain
detected detected
regionalExpression at the dorsal midline of the diencephalon and midbrain.
optic cup
strong strong
regionalSites of strong expression include the dorsal midline of neural tube (does not continue caudal to isthmus), optic cup, and otic vesicle (pigmented epithelium of the retina).
brain
strong strong
regionalSites of strong expression include the dorsal midline of neural tube (does not continue caudal to isthmus), optic cup, and otic vesicle (pigmented epithelium of the retina).
otocyst
strong strong
regionalSites of strong expression include the dorsal midline of neural tube (does not continue caudal to isthmus), optic cup, and otic vesicle (pigmented epithelium of the retina).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2136809
Entity Detected:Wnt2b, wingless related MMTV integration site 2b ( MGI:1261834)
Sequence:sense strand is shown

>MGI:2136809
CGGGCCTGCAGCCAGGGTGAGCTGAGCGTGTGCAGCTGTGACCCATATACCCGCGGTCGGCACCATGATC
AACGAGGGGACTTTGACTGGGGTGGCTGTAGTGACAACATCCATTACGGTGTTCGCTTTGCCAAGGCTTT
TGTGGATGCCAAAGAGAAGAGGCTTAAGGATGCCCGGGCCCTCATGAACTTACACAACAACCGCTGTGGT
CGCACGGCTGTTCGGAGATTCCTGAAGCTGGAGTGCAAGTGTCACGGTGTGAGTGGCTCCTGTACTCTGC
GCACCTGCTGGAGAGCACTCTCAGACTTCCGACGCACAGGTGACTACCTGAGGAGGCGATATGATGGGGC
TGTGCAGGTGACGGCCACACAGGATGGGGCCAATTTCACAGCAGCGCGCCAGGGCTATCGCCACGCCACC
CGGACTGATCTTGTCTACTTTGACAACTCCCCTGACTACTGTGTCTTGGACAAGGCTGCAGGTTCCCTAG
GTACCGCAGGCCGCGTCTGCAGCAAGACTTCTAAAGGAACAGATGGGTGTGAAATCATGTGTTGTGGCCG
AGGGTATGACACAACTCGGGTCACCCGCGTCACCCAGTGTGAGTGCAAATTCCACTGGTGCTGTGCTGTG
CGGTGCAAGGAGTGCAGAAACACTGTGGATGTCCACACATGCAAGGCCCCTAAGAAGGCAGAGTGGCTGG
ACCAGACCTGAACACACAGAAACCTCATTCTTCCCTCCACTTCAAGCCTCTGACTCAAAAGCACAAGACC
CTTGCATGCGCACCTTCCTCTACCCTCAATCCTGGGCTGCTATGGCTTCTGTCACGGACCTGGAGAGTGA
TCCGGAGGGACCCCAATGTCCCGGCCGCCTGGTTCCTTAGCCCTAGGGACGTGTTGATAGGGGATGGATT
TAGGAGGCTGAGTGACTCCCTGATGGTCCATCTGGAGGTTTGAAGGGAGAGTAGGAGAGGTCTGTCTTCA
GAGTGATTTGAGTTGCACTAA
nt 1 - nt 1001 of AF038384.1
Notes:The Wnt2b probe used in this study by Grove et al., 1998 [PMID:9584130] was described as being generated from cDNA clone pRK, a "1 kb insert in pEXlox vector; linearization by EcoRI digestion, antisense riboprobe transcription with SP6 polymerase." (GenBank submission AF038384).
Chemistry:RNA
Strand:antisense
Label:digoxigenin or fluorescein
Specimen
Organism:mouse
Strain:CD1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Grove et al., 1998 [PMID:9584130] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9584130] Grove EA, Tole S, Limon J, Yip L, Ragsdale CW 1998 The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development (125):2315-25
Links:MGI:2136855 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI