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EMAGE:3461

Ebf1 early B-cell factor 1 ( MGI:95275)
TS18 (11 dpc)
in situ hybridisation

Data Images
EMAGE:3461 EMAGE:3461
Fig 1A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.02.005] Gene Expr Patterns 4: 537-42, Mella S; Soula C; Morello D; Crozatier M; Vincent A, Expression patterns of the coe/ebf transcription factor genes during chicken and mouse limb development. Copyright 2004 Fig1B.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.02.005] Gene Expr Patterns 4: 537-42, Mella S; Soula C; Morello D; Crozatier M; Vincent A, Expression patterns of the coe/ebf transcription factor genes during chicken and mouse limb development. Copyright 2004

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: White line labelled B (in image A) indicates level from which section shown in B is taken. arrow in B = ebf expression in mesenchymal cells at the frontier between the trunk and limb bud. arrowhead in B = ebf expression in mesenchymal cells in the limb bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:3461EMAGE:3461EMAGE:3461Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3461_wholemount_strong_3D_2.wlz
3461_wholemount_moderate_3D_2.wlz
3461_wholemount_weak_3D_2.wlz
3461_wholemount_notDetected_3D_2.wlz
3461_voxel_strong_3D_1.wlz
3461_voxel_moderate_3D_1.wlz
3461_voxel_weak_3D_1.wlz
3461_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3461_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalExpression is in the limb and at the junction between the forming limb and the trunk.
trunk mesenchyme
detected detected
regionalExpression is at the junction between the forming limb and the trunk.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3052821
Entity Detected:Ebf1, early B-cell factor 1 ( MGI:95275)
Notes:The Ebf1 probe used in this study by Mella et al., 2004 [PMID:15261831] i indicated as that previously described by Hagman et al., 1995 [PMID:7796816] . Editors note: Several Ebf1 clones are described by Hagman and it is not specified which was used as template for the probe used in this study.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Embedding:albumin/gelatin
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Mella S; Soula C; Morello D; Crozatier M; Vincent A, 2004 [PMID:15261831] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2004.02.005] [ PMID:15261831] Mella S, Soula C, Morello D, Crozatier M, Vincent A 2004 Expression patterns of the coe/ebf transcription factor genes during chicken and mouse limb development. Gene Expr Patterns (4):537-42
 [ PMID:7796816] Hagman J, Gutch MJ, Lin H, Grosschedl R 1995 EBF contains a novel zinc coordination motif and multiple dimerization and transcriptional activation domains. EMBO J (14):2907-16
Links:MGI:3052822 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI