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EMAGE:3463

En1 engrailed 1 ( MGI:95389)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3463 EMAGE:3463
Fig3K.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00087-5] Gene Expr Patterns 3: 397-405, Zhou XH; Brandau O; Feng K; Oohashi T; Ninomiya Y; Rauch U; Fassler R, The murine Ten-m/Odz genes show distinct but overlapping expression patterns during development and in adult brain. Copyright 2003 Fig3L.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00087-5] Gene Expr Patterns 3: 397-405, Zhou XH; Brandau O; Feng K; Oohashi T; Ninomiya Y; Rauch U; Fassler R, The murine Ten-m/Odz genes show distinct but overlapping expression patterns during development and in adult brain. Copyright 2003

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: asterisk - mid/hindbrain junction; hb - hindbrain; arrow - apical ectoderm ridge; arrow head - anterior somites. Image L is a dorsal view of the embryo shown in image K.
Expression Pattern Description
Spatial Annotation:
EMAGE:3463Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3463_wholemount_strong_3D_1.wlz
3463_wholemount_possible_3D_1.wlz
3463_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3463_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalExpression is at the midbrain/hindbrain junction extending into the anterior part t of the hindbrain.
midbrain
detected detected
regionalExpression is at the midbrain/hindbrain junction extending into the anterior part t of the hindbrain.
forelimb bud apical ectodermal ridge
detected detected
hindlimb bud apical ectodermal ridge
detected detected
trunk somite
detected detected
regionalExpression is in anterior somites.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2158643
Entity Detected:En1, engrailed 1 ( MGI:95389)
Notes:The En1 probe used in this study by Zhou et al., [PMID:12915301] is indicated as that previously described by Millen et al., 1995 [PMID:8575294] as "a 600 bp EcoRI fragment of the 3' untranslated end of the cDNA (Joyner and Martin, 1987 [PMID:2892757] )." Editor's note: A 600bp EcoRI fragment could not be identified from the information in Joyner and Martin, or when EcoRI digesting the mouse En1 cDNA RefSeq clone NM 010133.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Zhou XH; Brandau O; Feng K; Oohashi T; Ninomiya Y; Rauch U; Fassler R, 2003 [PMID:12915301] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00087-5] [ PMID:12915301] Zhou XH, Brandau O, Feng K, Oohashi T, Ninomiya Y, Rauch U, Fassler R 2003 The murine Ten-m/Odz genes show distinct but overlapping expression patterns during development and in adult brain. Gene Expr Patterns (3):397-405
 [ PMID:8575294] Millen KJ, Hui CC, Joyner AL 1995 A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum. Development (121):3935-45
 [ doi:10.1101/gad.1.1.29] [ PMID:2892757] Joyner AL, Martin GR 1987 En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis. Genes Dev (1):29-38
Links:MGI:3606492 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI