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EMAGE:3464

Fgf6 fibroblast growth factor 6 ( MGI:95520)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3464
Fig1Q.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.04.009] Gene Expr Patterns 6: 94-101, Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Copyright 2005

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: White arrowheads - segmented expression in myotomes.
Expression Pattern Description
Spatial Annotation:
EMAGE:3464Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3464_wholemount_strong_3D_1.wlz
3464_wholemount_moderate_3D_1.wlz
3464_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3464_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalAuthors describe expression in the myotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9260
Entity Detected:Fgf6, fibroblast growth factor 6 ( MGI:95520)
Notes:The Fgf6 probe used in this study by Machka et al., 2005 [PMID:15979417] was a gift from P. Soriano (Tallquist et al., 2000 [PMID:11060232] ). The probe is described in the Tallquist et al. paper as being provided by G. Martin (deLapeyriere et al., 1993 [PMID:8223280] , where it is described as being "a 434 bp EcoRV fragment of the 'dream' plasmid containing the 3' untranslated part of Fgf6 cDNA (Ollendorff et al., 1992 [PMID:1477139] )." Editors Note: there are no EcoRV sites in the Fgf6 mouse cDNA RefSeq NM_010204.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, 2005 [PMID:15979417] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.04.009] [ PMID:15979417] Machka C, Kersten M, Zobawa M, Harder A, Horsch M, Halder T, Lottspeich F, Hrabe de Angelis M, Beckers J 2005 Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Gene Expr Patterns (6):94-101
 [ PMID:11060232] Tallquist MD, Weismann KE, Hellstrom M, Soriano P 2000 Early myotome specification regulates PDGFA expression and axial skeleton development. Development (127):5059-70
 [ PMID:8223280] deLapeyriere O, Ollendorff V, Planche J, Ott MO, Pizette S, Coulier F, Birnbaum D 1993 Expression of the Fgf6 gene is restricted to developing skeletal muscle in the mouse embryo. Development (118):601-11
 [ doi:10.1016/0300-9084(92)90025-A] [ PMID:1477139] Ollendorff V, Rosnet O, Marics I, Birnbaum D, deLapeyriere O 1992 Isolation and sequence of the murine Fgf6 cDNA. Biochimie (74):1035-8
Links:MGI:3617199 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI