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EMAGE:3475

Gbx2 gastrulation brain homeobox 2 ( MGI:95668)
TS14 (9.2 dpc)
in situ hybridisation

Data Images
EMAGE:3475
Fig2H.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00056-5] Gene Expr Patterns 3: 497-501, Acampora D; Annino A; Puelles E; Alfano I; Tuorto F; Simeone A, OTX1 compensates for OTX2 requirement in regionalisation of anterior neuroectoderm. Copyright 2003

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3475Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3475_wholemount_strong_3D_1.wlz
3475_wholemount_moderate_3D_1.wlz
3475_wholemount_possible_3D_1.wlz
3475_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3475_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalExpression is at the midbrain-hindbrain boundary.
future hindbrain
detected detected
regionalExpression is at the midbrain-hindbrain boundary.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1321259
Entity Detected:Gbx2, gastrulation brain homeobox 2 ( MGI:95668)
Sequence:sense strand is shown

>MGI:1321259
CCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGTACCGCCTTCAGCATAGACTCGCTGATCGGCA
GCCCGCCGCAGCCCAGTCCCGGCCATTTCGTCTACACCGGCTACCCCATGTTCATGCCCTACCGGCCGGT
GGTGCTGCCGCCACCGCCGCCACCGCCTCCCGCGCTGCCCCAGGCAGCGCTGCAGCCCGCTCTGCCGCCC
GCGCACCCTCACCACCAGATCCCCAGCCTGCCCACCGGCTTCTGCTCCAGCCTGGCGCAGGGCATGGCGC
TCACCTCCACGCTCATGGCCACTCTGCCCGGCGGCTTCTCTGCGTCGCCCCAGCACCAAGAGGCGGCGGC
TGCCCGCAAGTTCGCTCCACAGCCACTGCCCGGAGGCGGCAACTTCGACAAAGCCGAGGCGCTCCAAGCG
GATGCGGAAGACGGCAAAGCCTTCTTGGCCAAGGAGGGCTCGCTGCTCGCTTTCTCTGCGGCCGAAGCGG
TGCAGGCGTCGCTCGTCGGGGCTGTCCGAGGGCAAGGGAAAGACGAGTCAAAGGTGGAAGATGACCCGAA
GGGCAAGGAGGAGAGCTTCTCTCTGGAGAGCGATGTGGATTACAGCTCAGATGACAATTTGCCTGGTCAG
ACTGCTCATAAGGAAGAAGACCCCGGCCACGCACTGGAGGAGACCCCGCAGAGCGGCGGTGCAGCAGGCA
GCACCACGTCCACAGGCAAGAACCGGCGGCGGCGGACTGCCTTCACCAGCGAACAGCTGCTGGAGCTGGA
GAAAGAATTCCACTGCAAAAAGTACCTCTCCCTGACCGAGCGCTCACAGATCGCCCACGCCCTCAAACTC
AGCGAGGTGCAAGTAAAAATCTGG
nt 441 - nt 1304 of NM_010262.1
Notes:The Gbx2 probe used in this study by Acampora et al., 2003 [PMID:12915318] is indicated as that originally described in Acampora et al., 1998 [PMID:9811592] as a "PCR fragment including the region between aa 6 and aa 294". Editor's note: the co-ordinates shown above were deduced from this information.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.2 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Acampora D; Annino A; Puelles E; Alfano I; Tuorto F; Simeone A, 2003 [PMID:12915318] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00056-5] [ PMID:12915318] Acampora D, Annino A, Puelles E, Alfano I, Tuorto F, Simeone A 2003 OTX1 compensates for OTX2 requirement in regionalisation of anterior neuroectoderm. Gene Expr Patterns (3):497-501
 [ PMID:9811592] Acampora D, Avantaggiato V, Tuorto F, Briata P, Corte G, Simeone A 1998 Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation. Development (125):5091-104
 [ doi:10.1006/geno.1997.4969] [ PMID:9417909] Chapman G, Remiszewski JL, Webb GC, Schulz TC, Bottema CD, Rathjen PD 1997 The mouse homeobox gene, Gbx2: genomic organization and expression in pluripotent cells in vitro and in vivo. Genomics (46):223-33
Links:MGI:3045231 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI