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EMAGE:348

Wnt2b wingless related MMTV integration site 2b ( MGI:1261834)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:348 EMAGE:348 EMAGE:348 EMAGE:348 EMAGE:348
Figure 1B of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1A of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1D of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1E of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1F of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.
EMAGE:348
Figure 7I of Grove et al., 1998 [PMID:9584130] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: B: Medial view of a telencephalic hemisphere (rostral is to the left). cpe - choroid plexus epithelium; arrowheads marked D, E and F indicate the regions where sections D, E and F are taken from a similar hemisphere. A: Dorsal view of forebrain (rostral is to the left). t - telencephalon; d - diencephalon. D,E,F: (midline is to the left) cpe - choroid plexus epithelium. I: cpe - choroid plexus epithelium; hem - cortical hem; cpm - mesenchymal layer.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
telencephalon
strong strong
regionalStrong expression in a band along the caudal 2/3rds of the medial wall, adjacent to the lateral ventricle, defines the cortical hem. At rostral levels, band is dorsal to CPe; at caudal levels, band curves ventrally to surround caudal end of CPe. Expression marks the dorsal midline and two patches on either side of the midline. Expression marks the cortical hem; no expression in the junctional epithelium between the hem and CPe; abuts the CPe dorsally (Fig. 1D, 1E), caudally (Fig. 1F) and caudoventrally (Fig. 1E).
diencephalon
detected detected
regionalExpression marks the dorsal midline and two patches on either side of the midline.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2136809
Entity Detected:Wnt2b, wingless related MMTV integration site 2b ( MGI:1261834)
Sequence:sense strand is shown

>MGI:2136809
CGGGCCTGCAGCCAGGGTGAGCTGAGCGTGTGCAGCTGTGACCCATATACCCGCGGTCGGCACCATGATC
AACGAGGGGACTTTGACTGGGGTGGCTGTAGTGACAACATCCATTACGGTGTTCGCTTTGCCAAGGCTTT
TGTGGATGCCAAAGAGAAGAGGCTTAAGGATGCCCGGGCCCTCATGAACTTACACAACAACCGCTGTGGT
CGCACGGCTGTTCGGAGATTCCTGAAGCTGGAGTGCAAGTGTCACGGTGTGAGTGGCTCCTGTACTCTGC
GCACCTGCTGGAGAGCACTCTCAGACTTCCGACGCACAGGTGACTACCTGAGGAGGCGATATGATGGGGC
TGTGCAGGTGACGGCCACACAGGATGGGGCCAATTTCACAGCAGCGCGCCAGGGCTATCGCCACGCCACC
CGGACTGATCTTGTCTACTTTGACAACTCCCCTGACTACTGTGTCTTGGACAAGGCTGCAGGTTCCCTAG
GTACCGCAGGCCGCGTCTGCAGCAAGACTTCTAAAGGAACAGATGGGTGTGAAATCATGTGTTGTGGCCG
AGGGTATGACACAACTCGGGTCACCCGCGTCACCCAGTGTGAGTGCAAATTCCACTGGTGCTGTGCTGTG
CGGTGCAAGGAGTGCAGAAACACTGTGGATGTCCACACATGCAAGGCCCCTAAGAAGGCAGAGTGGCTGG
ACCAGACCTGAACACACAGAAACCTCATTCTTCCCTCCACTTCAAGCCTCTGACTCAAAAGCACAAGACC
CTTGCATGCGCACCTTCCTCTACCCTCAATCCTGGGCTGCTATGGCTTCTGTCACGGACCTGGAGAGTGA
TCCGGAGGGACCCCAATGTCCCGGCCGCCTGGTTCCTTAGCCCTAGGGACGTGTTGATAGGGGATGGATT
TAGGAGGCTGAGTGACTCCCTGATGGTCCATCTGGAGGTTTGAAGGGAGAGTAGGAGAGGTCTGTCTTCA
GAGTGATTTGAGTTGCACTAA
nt 1 - nt 1001 of AF038384.1
Notes:The Wnt2b probe used in this study by Grove et al., 1998 [PMID:9584130] was described as being generated from cDNA clone pRK, a "1 kb insert in pEXlox vector; linearization by EcoRI digestion, antisense riboprobe transcription with SP6 polymerase." (GenBank submission AF038384).
Chemistry:RNA
Strand:antisense
Label:digoxigenin or fluorescein
Specimen
Organism:mouse
Strain:CD1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Grove et al., 1998 [PMID:9584130] Indexed by GXD
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9584130] Grove EA, Tole S, Limon J, Yip L, Ragsdale CW 1998 The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development (125):2315-25
Links:MGI:2136855 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI