EMAGE mouse gene expression spatial database

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EMAGE:3482

Lhx2 LIM homeobox protein 2 ( MGI:96785)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig3C.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.03.001] Gene Expr Patterns 4: 521-8, Kolterud A; Wandzioch E; Carlsson L, Lhx2 is expressed in the septum transversum mesenchyme that becomes an integral part of the liver and the formation of these cells is independent of functional Lhx2. Copyright 2004

Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3482_voxel_strong_3D_1.wlz

3482_voxel_moderate_3D_1.wlz

3482_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3482_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3052960
Entity Detected:	Lhx2, LIM homeobox protein 2 ( MGI:96785)
Sequence:	sense strand is shown >MGI:3052960 AACTCACCTGCTTCAGCAAGGATGGCAGCATCTACTGCAAAGAAGACTACTACAGGCGGTTCTCTGTGCA GCGCTGCGCCCGCTGCCACCTGGGCATCTCGGCCTCAGAGATGGTGATGCGCGCTCGGGACTTGGTTTAT CACCTCAACTGCTTCACATGCACAACGTGTAACAAGATGCTGACGACCGGCGACCATTTCGGCATGAAGG ACAGCCTGGTCTATTGCCGCTTGCACTTCGAGGCTCTGCTGCAGGGCGAATACCCAGCACACTTTAACCA TGCCGACGTGGCAGCGGCGGCAGCCGCAGCCGCAGCAGCTAAGAGTGCAGGATTGGGCTCAGCCGGGGCT AATCCGCTGGGTCTTCCCTACTACAACGGCGTGGGCACTGTGCAAAAGGGGAGGCCGAGAAAGCGCAAGA GTCCAGGACCCGGGGCAGATCTGGCAGCTTACAACGCCGCGCTTAGCTGTAACGAGAACGATGCTGAACA CCTGGATCGTGACCAGCCCTACCCCAGCAGCCAAAAGACAAAGCGCATGCGCACCTCCTTCAAGCACCAC CAGCTTCGGACAATGAAGTCTTACTTTGCCATTAACCACAATCCGGATGCCAAGGACTTGAAGCAGCTTG CGCAAAAGACCGGCCTCACCAAGAGAGTCCTCCAGGTCTGGTTTCAGAATGCCCGGGCCAAGTTCAGGCG CAACCTTTTACGGCAGGAAAACACGGGCGTGGACAAGACGTCAGATGCCACGCTGCAGACAGGGACGCCG TCAGGGCCCGCCTCGGAGCTGTCCAACGCCTCGCTCAGCCCCTCCAGCACGCCTACAACCCTCACAGACT TGACTAGCCCCACCCTGCCGACTGTGACGTCAGTCTTAACTTCTGTGCCTGGCAACCTGGAGGGCCACGA GCCCCACAGCCCTTCACAAACGACTCTTACCAACCTTTTCTAATGACTCGCCACCCCCTTCTCCCCGAGC CCCCACGATTTCTTTAAAAAAGAAATTATCTTTAGTTGAATTCCAAGTGTATTTTAAAAATAGAGGTTTG AGCAACTAACTAACCACGTTTTAGGATCTCGCCTGGAAACAGAGGGAAAAAAGAATTGTGCGTCGGGCTA ACGCAGCGGTGTGTGCTGAGGAATTACTTGGGAGATATATCTGCAACACAACATTTGTGTCCCTGTACAG TTTTGTGGACTGAGCGAGGAAAACAACAAATAATTTAAGTTGGCTGAGAGCTTCCGTATTTTCAAAGACT GCCACGTGCCTTAGGAATACTGTTTTATCTT
	nt 460 - nt 1750 of NM_010710.1
Notes:	The Lhx2 probe used in this study by Kolterud et al., 2004 [PMID:15261829] is described as being "synthesized from cDNA fragment obtained by rtPCR. The following template was used: Lhx2 (NM_010710 nucleotides 460-1750)." Editors Note: The authors do not refer to a version of NM_010710 and as of Feb 2007 there were two versions: NM_010710.1 (1878bp) and NM_010710.2 (2289bp). However, as NM_010710.2 was released on 03-DEC-2006 (i.e. after publication date of this paper), the co-ordinates given presumably refer to NM_010710.1.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	section

Procedures

Staining procedure:

alkaline phosphatase + NBT/BCIP

General Information

Authors:	Kolterud A; Wandzioch E; Carlsson L, 2004 [PMID:15261829] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2004.03.001] [ PMID:15261829] Kolterud A, Wandzioch E, Carlsson L 2004 Lhx2 is expressed in the septum transversum mesenchyme that becomes an integral part of the liver and the formation of these cells is independent of functional Lhx2. Gene Expr Patterns (4):521-8
Links:	MGI:3052985 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source