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EMAGE:3521

Nkx2-2 NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347)
TS12 (6 Somite no.)
in situ hybridisation

Data Images
EMAGE:3521 EMAGE:3521 EMAGE:3521
Fig4E.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998. Figure 4F of Pabst et al., 1998 [PMID:9545546] . Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998. Figure 4G of Pabst et al., 1998 [PMID:9545546] . Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00035-5] Mech Dev 73: 85-93, Pabst O; Herbrand H; Arnold HH, Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Copyright 1998.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Parallel double in situ hybridizations on carefully staged embryos. Nkx2-9 expression (red) appears to extend far more caudally and also more dorsally than Nkx2-2 (blue) on transverse sections through the head (E,F) and trunk (G) of a six-somite stage embryo. ht, heart; pfb, prospective forebrain; phb, prospective hindbrain; pmb, prospective midbrain; so, somite. ***Only the Nkx2-2 expression has been annotated in this EMAGE entry***
Expression Pattern Description
Spatial Annotation:
EMAGE:3521EMAGE:3521EMAGE:3521EMAGE:3521Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3521_voxel_possible_3D_1.wlz
3521_voxel_notDetected_3D_1.wlz
3521_voxel_moderate_3D_1.wlz
3521_voxel_moderate_3D_2.wlz
3521_voxel_possible_3D_2.wlz
3521_voxel_notDetected_3D_2.wlz
3521_voxel_strong_3D_1.wlz
3521_voxel_possible_3D_3.wlz
3521_voxel_notDetected_3D_3.wlz
3521_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3521_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future prosencephalon
detected detected
regionalExpression is in a domain and overlaps with Nkx2-9 expression as seen in MGI:3029967/EMAGE:3855.
future hindbrain
detected detected
regionalExpression is in a domain and overlaps with Nkx2-9 expression as seen in MGI:3029967/EMAGE:3855.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1270231
Entity Detected:Nkx2-2, NK2 transcription factor related, locus 2 (Drosophila) ( MGI:97347)
Sequence:sense strand is shown

>MGI:1270231
TGGACGCTGTGCAGAGCCTGCCCCTTAAGAGCCCTTTCTACGACAGCAGCGACAACCCCTACACTCGCTG
GCTGGCCAGCACCGAGGGCCTCCAATACTCCCTGCACGGGCTGGCGGCCAGCGCTCCCCCCCAAGACTCG
AGCTCCAAGTCCCCAGAGCCCTCGGCTGACGAGTCACCGGACAATGACAAGGAGACCCAGGGCGGCGGGG
GGGACGCAGGCAAGAAGCGGAAGCGCCGAGTGCTCTTCTCCAAAGCGCAGACCTACGAGCTGGAGCGGCG
CTTCCGGCAGCAGCGGTACCTGTCGGCGCCCGAGCGCGAGCACCTGGCCAGCCTCATCCGTCTCACGCCG
ACACAGGTCAAGATCTGGTTCCAGAACCATCGCTACAAGATGAAACGTGCCCGGGCGGAGAAAGGTATGG
AGGTGACGCCTCTGCCCTCGCCGCGCCGTGTGGCAGTGCCGGTCTTGGTCAGGGACGGCAAACCGTGCCA
CGCGCTCAAAGCCCAGGACCTG
nt 501 - nt 1012 of NM_010919.1
Notes:The Nkx2-2 probe used in this study by Pabst et al., 1998 [PMID:9545546] was generated by RT-PCR using primers as follows: "Nkx2-2 upstream primer: TGGACGCTGTGCAGAGCCTG; Nkx2-2 downstream primer: CAGGTCCTGGGCTTTGAGCG" from total RNA isolated from "dissected parts of E9.5 mouse embryos and organs of fetal and adult mice".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:6 Somite no.
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Pabst O; Herbrand H; Arnold HH, 1998 [PMID:9545546] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(98)00035-5] [ PMID:9545546] Pabst O, Herbrand H, Arnold HH 1998 Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev (73):85-93
Links:MGI:3032438 same experiment
 EMAGE:3855 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE