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EMAGE:3526

Nkx2-6 NK2 transcription factor related, locus 6 (Drosophila) ( MGI:97351)
TS15 (9 dpc)
in situ hybridisation

Data Images
EMAGE:3526 EMAGE:3526
Fig2B.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00174-3] Mech Dev 69: 215-8, Nikolova M; Chen X; Lufkin T, Nkx2.6 expression is transiently and specifically restricted to the branchial region of pharyngeal-stage mouse embryos Copyright 1997. Fig2C.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00174-3] Mech Dev 69: 215-8, Nikolova M; Chen X; Lufkin T, Nkx2.6 expression is transiently and specifically restricted to the branchial region of pharyngeal-stage mouse embryos Copyright 1997.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Arrows indicate the branchial arch regions. Abbreviations: h, heart; o, otic vesicle; 1, first branchial arch.
Expression Pattern Description
Spatial Annotation:
EMAGE:3526EMAGE:3526EMAGE:3526Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3526_voxel_notDetected_3D_1.wlz
3526_voxel_strong_3D_1.wlz
3526_voxel_notDetected_3D_2.wlz
3526_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3526_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component
not detected not detected
homogeneous
1st branchial arch maxillary component
not detected not detected
homogeneous
2nd branchial arch
detected detected
3rd branchial arch
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1196880
Entity Detected:Nkx2-6, NK2 transcription factor related, locus 6 (Drosophila) ( MGI:97351)
Sequence:sense strand is shown

>MGI:1196880
GTCGGTGCACCTGGGAGACAGTCCTGGAGATGGGCTCGAATCCTGTGGGGGAGCCACGTAAAAACTCCTC
CGGGCACGATCTCCCGACTTGGAGCCAGGAACCCAATGACCGATCGCGGCGTGGGCAACCTGAGTGGCGA
CATGCGCCGAGGCGGTCCGGTTTCAACCAGAACGCGGCCACAGCGGAAGTCCCGCGTGCTATTCTCCCAG
GCCCAAGTGCTGGCCCTGGAGCGGCGCTTCAAGCAGCAGCGATACCTGACTGCGCCCGAGCGTGAGCACT
TGGCCAGCGCGCTGCAGCTCACGTCCACGCAGGTCAAGATCTGGTTCCAAAACCGGCGCTACAAGTCCAA
GAGTCAGCGCCAGGACCAGAATCTGGAACTGGCCGGCCACCCGTTAGCACCGCGCCCGGGTAGCAGTGCC
AGTACTGGTACTGGACGGCAACCCCTGCCTGGATCCGACGTAGCCGCATTCCTGGTCCCTACAAAGCCAC
CTCGCCCTATTCCTGCTTCGGTGGCTACGCGGGCACTCCCTACGACGCTAGCTATGCGAGCCGCTGCACC
AGCGCCAGCGCCGGCCCCGGGCCGCTCACACCACTGGCCAGCTCTGGCTTCAGCCCAGGTGGCCAAAGTG
CGGCTCCGCAGGGCCATCTGCCCGCTACGCCTCAGGGAGTCACGGCCTGGTGAAAAGCCTGAGCTCACCT
ACTGCCATTCGGTGCCTGATGCCTGGTCCCCTCCCCTTCCTGCTGGTGGGCGCGGGGCTGGAAAGCACTG
CCCACCTTACTGATCTCGGAACTGAACTGCTTGGAGGAGCGGCCCTGAAGACTTGAGAACATTCTTTCCC
TCGGAAGTGTAACCACGTAGCCACGTAGATGCTGTGCAGAAAGACTAGCACAAGAAATTAATAATCTATC
CCTGCCTCTGCTAGAAAGTAAAAAAAAAAAAAAA
nt 1 - nt 944 of AF030113.1
Notes:The Nkx2-6 probe used in this study by Nikolova et al., 1997 [PMID:9486544] was transcribed from the insert of the EST clone AA108980. The authors sequenced the insert of this clone in its entireity and deposited this sequence to GenBank as AF030113.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6J
Age:9 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:araldite
Staining procedure:alkaline phosphatase + Purple Precipitating Reagent
General Information
Authors:Nikolova M; Chen X; Lufkin T, 1997 [PMID:9486544] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(97)00174-3] [ PMID:9486544] Nikolova M, Chen X, Lufkin T 1997 Nkx2.6 expression is transiently and specifically restricted to the branchial region of pharyngeal-stage mouse embryos. Mech Dev (69):215-8
Links:MGI:2429957 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI