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EMAGE:3542

Prox1 prospero-related homeobox 1 ( MGI:97772)
TS14 (15-18 Somite no.)
in situ reporter

Data Images
EMAGE:3542
Fig1F.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00240-X] Mech Dev 118: 147, Burke Z; Oliver G, Prox1 is an early specific marker for the developing liver and pancreas in the mammalian foregut endoderm. Copyright 2002.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrow indicates the node. Black, Red, and Yellow arrowheads indicate the liver bud, hindgut and dorsal pancreas, respectively.
Expression Pattern Description
Spatial Annotation:
EMAGE:3542Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3542_wholemount_strong_3D_1.wlz
3542_wholemount_moderate_3D_1.wlz
3542_wholemount_weak_3D_1.wlz
3542_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3542_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
biliary bud
detected detected
Expression is present in the liver bud.
foregut-midgut junction
detected detected
regionalExpression is present in the dorsal pancreas.
hindgut diverticulum
detected detected
regionalExpression is present in the hindgut near the region of the node.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Prox1, prospero-related homeobox 1 ( MGI:97772)
Notes:Prox1 expression was detected in this study by Burke & Oliver, 2002 [PMID:12351178] thus: "We have previously reported the inactivation of the mouse Prox1 gene by the in-frame insertion of the lacZ gene. The lacZ gene, which encodes beta-Gal, was inserted at amino acid 224 of Prox1 (Wigle et al., 1999 [PMID:10080188] ). The resulting lacZ activity allowed us to easily detect cells that normally express Prox1 throughout development."
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129S1/Sv * 129X1/SvJ * NMRI
Age:15-18 Somite no.
Theiler Stage:TS14
Mutations:Prox1tm1Gco/Prox1+
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Burke Z & Oliver G, 2002 [PMID:12351178] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(02)00240-X] [ PMID:12351178] Burke Z, Oliver G 2002 Prox1 is an early specific marker for the developing liver and pancreas in the mammalian foregut endoderm. Mech Dev (118):147-55
 [ doi:10.1038/6844] [ PMID:10080188] Wigle JT, Chowdhury K, Gruss P, Oliver G 1999 Prox1 function is crucial for mouse lens-fibre elongation. Nat Genet (21):318-22
Links:MGI:3529572 same experiment
 MGI:2386336 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI