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EMAGE:3548

Shh sonic hedgehog ( MGI:98297)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3548
Fig3A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00029-X] Mech Dev 73: 59-72, Greene ND; Gerrelli D; Van Straaten HW; Copp AJ, Abnormalities of floor plate, notochord and somite differentiation in the loop-tail (Lp) mouse: a model of severe neural tube defects. Copyright 1998.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: f, foregut; h, hindgut.
Expression Pattern Description
Spatial Annotation:
EMAGE:3548Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3548_wholemount_strong_3D_1.wlz
3548_wholemount_moderate_3D_1.wlz
3548_wholemount_weak_3D_1.wlz
3548_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3548_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
notochord
detected detected
Expression is present along its entire length.
diencephalon floor plate
detected detected
future midbrain floor plate
detected detected
Expression is present along its entire length.
future hindbrain
detected detected
regionalExpression is present along entire length of floor plate.
neural tube floor plate
detected detected
Expression is present along its entire length.
foregut
detected detected
hindgut
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Greene et al., 1998 [PMID:9545534] is indicated as that originally described by Echelard et al., 1993 [PMID:7916661] i.e. as "either a 2.8kb or 0.6kb RNA transcript" derived from "subclones of the original Shh cDNA inserts".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:involves: CBA/Ca * LPT/Le
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Greene ND; Gerrelli D; Van Straaten HW; Copp AJ, 1998 [PMID:9545534] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(98)00029-X] [ PMID:9545534] Greene ND, Gerrelli D, Van Straaten HW, Copp AJ 1998 Abnormalities of floor plate, notochord and somite differentiation in the loop-tail (Lp) mouse: a model of severe neural tube defects. Mech Dev (73):59-72
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:3529940 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI