EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3552

T brachyury ( MGI:98472)
TS14 (9.5 dpc)
in situ hybridisation

Data Images


Fig4B(Lp/+).Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(98)00029-X] Mech Dev 73: 59-72, Greene ND; Gerrelli D; Van Straaten HW; Copp AJ, Abnormalities of floor plate, notochord and somite differentiation in the loop-tail (Lp) mouse: a model of severe neural tube defects. Copyright 1998.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: Arrow indicates expression in notochord.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3552_wholemount_strong_3D_1.wlz

3552_wholemount_moderate_3D_1.wlz

3552_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3552_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

notochord

detected

Heterozygotes exhibit an intermediate level of expression compared to wild type and homozygotes.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2449092
Entity Detected:	T, brachyury ( MGI:98472)
Sequence:	sense strand is shown >MGI:2449092 GGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGG AGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAA GAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAG GGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGC TAACTAACGAGATGATTGTGACCAAGAACGGCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGG CCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATAT GTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAG ACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAA GCTCAATGGAGGGGGACAGATCATGTTAAACTCCTTGCATAAGTATGAACCTCGGATTCACATCGTGAGA GTTGGGGGCCCGCAACGCATGATCACCAGCCACTGCTTTCCCGAGACCCAGTTCATAGCTGTGACTGCCT ACCAGAATGAGGAGATTACAGCCCTTAAAATTAAATACAACCCATTTGCTAAAGCCTTCCTTGATGCCAA AGAAAGAAACGACCACAAAGATGTAATGGAGGAACCGGGGGACTGCCAGCAGCCGGGGTATTCCCAATGG GGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGC TCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCC CAGCCCCTATGCTCATCGGAACAGCTCTCCAACCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTG CAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACG CCAGCCCACCTACTGGCTCTAGCCAGTATCCCAGTCTCTGGTCTGTGAGCAATGGTACCATCACCCCAGG CTCCCAGACAGCTGGGGTGTCCAACGGGCTGGGAGCTCAGTTCTTTCGAGGCTCCCCTGCACATTACACA CCACTGACGCACACGGTCTCAGCTGCCACGTCCTCGTCTTCTGGTTCTCCGATGTATGAAGGGGCTGCTA CAGTCACAGACATTTCTGACAGCCAGTATGACACGGCCCAAAGCCTCCTCATAGCCTCGTGGACACCTGT GTCACCCCCATCTATGTGAATTGAACTTTCCTCCATGTGCTGAGACTTGTAACAACCGGTGTCAACTGGA TCTTCTAGGCTCAAAGTGGCAGGCTCTTGGGACAAGGGAAAAATAAATAAATAAAAGCTAGATACTAACA ACTCCATTTTCAAATAAGAGCAATAATACATGTCCTATAATCATGTTCTACAGCCTCTTGTTTGATACCT ACAGTAGTGATATGTGTCCTACATTATGAAGCCAAGGACAGAGAGACGGCTGTGGTCCAGTTTTTTGTGA CTGGCAGTTAATCAGAGTCCTTTGCTAGGTAGGGTCCTATATCTTGTGTTTCTCTACAACATATATGTGA CTTTGAAATCCTGG
	nt 1 - nt 1764 of X51683.1
Notes:	The T (Brachury) probe used in this study by Greene et al, 1998 [PMID:9545534] is indicated as that used by Herrmann, 1991 [PMID:1821859] i.e. "the sequence from base 1 to base 1764 of the cDNA for the T gene, pme75 (Herrmann et al., 1990 [PMID:2154694] )."
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	involves: CBA/Ca * LPT/Le
Age:	9.5 dpc
Theiler Stage:	TS14
Mutations:	Vangl2^Lp/Vangl2⁺
Preparation:	wholemount

Procedures

General Information

Authors:	Greene ND; Gerrelli D; Van Straaten HW; Copp AJ, 1998 [PMID:9545534] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(98)00029-X] [ PMID:9545534] Greene ND, Gerrelli D, Van Straaten HW, Copp AJ 1998 Abnormalities of floor plate, notochord and somite differentiation in the loop-tail (Lp) mouse: a model of severe neural tube defects. Mech Dev (73):59-72
	[ PMID:1821859] Herrmann BG 1991 Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development (113):913-7
Links:	MGI:3529945 same experiment
	MGI:1857642 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source