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EMAGE:3558

Nfe2l1 nuclear factor, erythroid derived 2,-like 1 ( MGI:99421)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:3558
Fig1A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: A, the anterior of the embryo; xe, extraembryonic tissue; e, embryonic tissue; ps, primitive streak.
Expression Pattern Description
Spatial Annotation:
EMAGE:3558Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3558_wholemount_strong_3D_1.wlz
3558_wholemount_moderate_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3558_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
There is uniform expression with an accumulation of transcripts at the boundary between embryo proper and the extra-embryonic tissues.
extraembryonic component
detected detected
There is uniform expression with an accumulation of transcripts at the boundary between embryo proper and the extra-embryonic tissues.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3033622
Entity Detected:Nfe2l1, nuclear factor, erythroid derived 2,-like 1 ( MGI:99421)
Notes:The Nfe2l1 (Tcf11) probe used in this study by Murphy & Kolsto, 2000 [PMID:11025215] was one of four possible probes: "(1) from a SacI site (accession no. X78709; bp no. 1444) to the end of the cDNA clone. (2) a SacI/HindIII fragment (1444-2098 bp). (3) from the HindIII site to a HincII site in the 3' non-coding region (2098-3434 bp). (4) A 535 bp extreme 5' probe was generated by RT-PCR." Editors note: These probes have been indexed by the MGI as MGI:3033622, MGI:3033623, MGI:3033624 and MGI:3033625 respectively, but it is not stipulated which was actually used for this particular assay.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Murphy P & Kolsto A, 2000 [PMID:11025215] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9753] [ PMID:10882515] Christoffels VM, Habets PE, Franco D, Campione M, de Jong F, Lamers WH, Bao ZZ, Palmer S, Biben C, Harvey RP, Moorman AF 2000 Chamber formation and morphogenesis in the developing mammalian heart. Dev Biol (223):266-78
Links:MGI:3033630 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI