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EMAGE:3559

Nfe2l1 nuclear factor, erythroid derived 2,-like 1 ( MGI:99421)
TS12 (8.25 dpc)
in situ hybridisation

Data Images
EMAGE:3559
Figure 1C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00413-5] Mech Dev 97: 141-8, Murphy P; Kolsto A, Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development Copyright 2000.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: mb, midbrain; fb, forebrain; hfm, headfold mesenchyme; h, heart.
Expression Pattern Description
Spatial Annotation:
EMAGE:3559Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3559_wholemount_strong_3D_1.wlz
3559_wholemount_moderate_3D_1.wlz
3559_wholemount_weak_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3559_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
heart
detected detected
head mesenchyme
detected detected
regionalExpression is in the mesenchyme of the head fold.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3033622
Entity Detected:Nfe2l1, nuclear factor, erythroid derived 2,-like 1 ( MGI:99421)
Notes:The Nfe2l1 (Tcf11) probe used in this study by Murphy & Kolsto, 2000 [PMID:11025215] was one of four possible probes: "(1) from a SacI site (accession no. X78709; bp no. 1444) to the end of the cDNA clone. (2) a SacI/HindIII fragment (1444-2098 bp). (3) from the HindIII site to a HincII site in the 3' non-coding region (2098-3434 bp). (4) A 535 bp extreme 5' probe was generated by RT-PCR." Editors note: These probes have been indexed by the MGI as MGI:3033622, MGI:3033623, MGI:3033624 and MGI:3033625 respectively, but it is not stipulated which was actually used for this particular assay.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:8.25 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Murphy P & Kolsto A, 2000 [PMID:11025215] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00413-5] [ PMID:110] Murphy P, Kolsto, A 2000 Expression of the bZIP transcription factor TCF11 and its potential dimerization partners during development. Mech Dev (97):141-8
Links:MGI:3033630 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI