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EMAGE:3577

Prdm1 PR domain containing 1, with ZNF domain ( MGI:99655)
TS11 (7.0 dpc)
immunohistochemistry

Data Images
EMAGE:3577 EMAGE:3577
Fig1A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00189-2] Mech Dev 117: 305-9, Chang DH; Calame KL, The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Copyright 2002 Fig1B.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00189-2] Mech Dev 117: 305-9, Chang DH; Calame KL, The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Copyright 2002

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Fig 1A shows immunostaining using the anti-Prdm1(Blimp-1) 3H2E8 antisera and Fig 1B shows isotype-matched mouse IgG1 antibody standard unlabeled (Southern Biotech) on an adjecent section to Fig 1A, which was used here as a negative control. Image annotation: ne - neural ectoderm, ac - amniotic cavity, ps - primitive streak, all - allantois.
Expression Pattern Description
Spatial Annotation:
EMAGE:3577EMAGE:3577Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3577_voxel_strong_3D_1.wlz
3577_voxel_moderate_3D_1.wlz
3577_voxel_possible_3D_1.wlz
3577_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3577_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo mesoderm
detected detected
regionalExpression is present in the head process region
embryo
detected detected
regionalExpression is present in the primordial germ cells
definitive endoderm
detected detected
regionalExpression is present in the head process region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:3603667
Entity Detected:Prdm1, PR domain containing 1, with ZNF domain ( MGI:99655)
Antigen:sense strand is shown

>MGI:3603667
SAREQNLAACQNGMNIYFYTIKPIPANQELLVWYCRDFAERLHYPYPGELTVINLTQTESNPKQYSSEKN
ELYPKSVPKREYSVKEILKLDSNPSKRKDIYRSNISPFTLEKDMDGFRKNGSPDMPFYPRVVYPIRAPLP
EDFLKASLAYGMERPTYITHSPLPSSTTPSPPASSSPEQSLKSSSPHSSPGNTVSPLAPGLPEHRDSYSY
L
aa 199 - aa 409 of AAA19252.1
Notes:The anti-Prdm1(Blimp-1) antibody used in this study by Chang & Calame (2002) [PMID:12204275] is described as "The monoclonal antibody, 3H2E8", "obtained after immunization with a peptide (amino acids 199-409) from mouse Blimp-1. The specificity of 3H2E8 was confirmed by positive immunocytostaining of endogenous Blimp-1 and ectopically expressed Blimp-1 in cells and negative staining following pre-absorption of 3H2E8 with Blimp-1 peptide (amino acids 199-409)"
Antibody Type:monoclonal
Catalogue Number:3H2E8
Specimen
Organism:mouse
Strain:either: BALB/c or C57BL/6
Age:7.0 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Secondary Antibody:anti-mouse IgG1
Labelled with:alkaline phosphatase
Visualisation method:NBT+BCIP
General Information
Authors:Chang DH; Calame KL (2002) [PMID:12204275] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(02)00189-2] [ PMID:12204275] Chang DH, Calame KL 2002 The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech Dev (2002):305-9
Links:MGI:3603675 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI