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EMAGE:3591

Cst3 cystatin C ( MGI:102519)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:3591 EMAGE:3591 EMAGE:3591 EMAGE:3591
Fig 3C.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig 3D.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig3A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig3B.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: Approximate location of sections is shown in (A). lv, left ventricle; m, myocardium; ot, outflow tract; rv, right ventricle. Arrows indicate relative orientations of the dorsal-ventral and right-left embryonic axes.
Expression Pattern Description
Spatial Annotation:
EMAGE:3591EMAGE:3591EMAGE:3591EMAGE:3591Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3591_wholemount_strong_3D_3.wlz
3591_wholemount_possible_3D_1.wlz
3591_wholemount_notDetected_3D_1.wlz
3591_voxel_strong_3D_1.wlz
3591_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3591_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive ventricle
detected detected
regionalMost notable expression is in the outer curvature (Fig3A,B). Expression is in the outer curvature in more caudal regions of the left ventricle (Fig3D).
outflow tract
detected detected
regionalExpression is in the myocardium of the outflow tract and in the truncus arteriosus of the proximal outflow tract (Fig3C).
primitive ventricle cardiac muscle
detected detected
See Fig3C.
heart
detected detected
regionalExpression is in the developing trabeculae. (Fig3C)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2671251
Entity Detected:Cst3, cystatin C ( MGI:102519)
Notes:The Cst3 probe used in this study by Smart et al, 2002 [PMID:14516662] is described as a 0.362kb RDA (representational difference analysis) clone. The exact sequence is not reported.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Smart N; Hill AA; Cross JC; Riley PR (2002) [PMID:14516662] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(03)00093-5] [ PMID:14516662] Smart N, Hill AA, Cross JC, Riley PR. 2002 A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis Mech Dev (119 Suppl 1):S65-71
Links:MGI:2671345 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI