EMAGE mouse gene expression spatial database

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EMAGE:3597

Pdx1 pancreatic and duodenal homeobox 1 ( MGI:102851)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Fig5E.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00240-X] Mech Dev 118: 147, Burke Z; Oliver G, Prox1 is an early specific marker for the developing liver and pancreas in the mammalian foregut endoderm. Copyright 2002	Fig6E.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00240-X] Mech Dev 118: 147, Burke Z; Oliver G, Prox1 is an early specific marker for the developing liver and pancreas in the mammalian foregut endoderm. Copyright 2002

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

The section in 6E is taken from the wholemount in 5E. Image annotations: In 5E and 6E: ventral (yellow arrow) and dorsal (yellow arrowhead) pancreatic buds. In 6E: DP - dorsal pancreatic bud; VP - ventral pancreatic buds; HE - hepatic bud.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3597_wholemount_strong_3D_1.wlz

3597_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3597_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

detected

regional

Expression is present in the ventral and dorsal pancreatic buds.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3529564
Entity Detected:	Pdx1, pancreatic and duodenal homeobox 1 ( MGI:102851)
Sequence:	sense strand is shown >MGI:3529564 CGCGGCCACACAGCTCTACAAGGACCCGTGCGCATTCCAGAGGGGCCCGGTGCCAGAGTTCAGCGCTAAC CCCCCTGCGTGCCTGTACATGGGCCGCCAGCCCCCACCTCCGCCGCCACCCCAGTTTACAAGCTCGCTGG GATCACTGGAGCAGGGAAGTCCTCCGGACATCTCCCCATACGAAGTGCCCCCGCTCGCCTCCGACGACCC GGCTGGCGCTCACCTCCACCACCACCTTCCAGCTCAGCTCGGGCTCGCCCATCCACCTCCCGGACCTTTC CCGAATGGAACCGAGCCTGGGGGCCTGGAAGAGCCCAACCGCGTCCAGCTCCCTTTCCCGTGGATGAAAT CCACCAAAGCTCACGCGTGGAAAGGCCAGTGGGCAGGAGGTGCTTACACAGCGGAACCCGAGGAAAACAA GAGGACCCGTACTGCCTACACCCGGGCGCAGCTGCTGGAGCTGGAGAAGGAATTCTTATTTAACAAATAC ATCTCCCGGCCCCGCCGGGTGGAGCTGGCAGTGATGTTGAACTTGACCGAGAGACACATCAAAATCTGGT TCCAAAACCGTCGCATGAAGTGGAAAAAAGAGGAAGATAAGAAACGTAGTAGCGGGACCCCGAGTGGGGG CGGTGGGGGCGAAGAGCCGGAGCAAGATTGTGCGGTGACCTCGGGCGAGGAGCTGCTGGCAGTGCCACCG CTGCCACCTCCCGGAGGTGCCGTGCCCCCAGGCGTCCCAGCTGCAGTCCGGGAGGGCCTACTGCCTTCGG GCCTTAGCGTGTCGCCACAGCCCTCCAGCATCGCGCCACTGCGACCGCAGGAACCCCGGTGAGGACAGCA GTCTGAGGGTGAGCGGGTCTGGGACCCAGAGTGTGGA
	nt 137 - nt 1013 of NM_008814.2
Notes:	The Pdx1 construct used for in vitro transcription of RNA probes in this study by Burke & Oliver, 2002 [PMID:12351178] "was generated by subcloning a 800 bp PCR fragment" (using the primers Pdx1-1 5'CACAAGCTTGCGGCCACACAGCTCTAC3' and Pdx1-2 5'GAGGGATCCACACTCTGGGTCCCAGAC3') "amplified from the full length cDNA sequence". Editors Note: These primers contain cloning sites on the 5'ends. The co-ordinates with reference to the Pdx1 cDNA RefSeq NM_008814.2 were deduced from the given information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Burke Z; Oliver G (2002) [PMID:12351178] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(02)00240-X] [ PMID:12351178] Burke Z, Oliver G 2002 Prox1 is an early specific marker for the developing liver and pancreas in the mammalian foregut endoderm. Mech Dev (118):147-55
Links:	MGI:3529584 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source