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EMAGE:3603

Bmp7 bone morphogenetic protein 7 ( MGI:103302)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:3603
Fig2e.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00515-3] Mech Dev 100: 165-75, Bulchand S; Grove EA; Porter FD; Tole S, LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem. Copyright 2001

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotation: hem, cortical hem.
Expression Pattern Description
Spatial Annotation:
EMAGE:3603EMAGE:3603Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3603_voxel_strong_3D_1.wlz
3603_voxel_moderate_3D_1.wlz
3603_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3603_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
choroid invagination
detected detected
Authors report expression in the choroid plexus.
telencephalon
detected detected
regionalExpression in dorsal telencephalon in cortical hem.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1275942
Entity Detected:Bmp7, bone morphogenetic protein 7 ( MGI:103302)
Sequence:sense strand is shown

>MGI:1275942
TCTTCAGTACCCAGGGCCCCCCTTTAGCCAGCCTGCAGGACAGCCACTTCCTCACTGACGCCGACATGGT
CATGAGCTTCGTCAACCTAGTGGAACATGACAAAGAATTCTTCCACCCTCGATACCACCATCGGGAGTTC
CGGTTTGATCTTTCCAAGATCCCCGAGGGCGAAGCGGTGACCGCAGCCGAATTCAGGATCTATAAGGACT
ACATCCGGGAGCGATTTGACAACGAGACCTTCCAGATCACAGTCTATCAGGTGCTCCAGGAGCACTCAGG
CAGGGAGTCGGACCTCTTCTTGCTGGACAGCCGCACCATCTGGGCTTCTGAGGAGGGCTGGTTGGTGTTT
GATATCACAGCCACCAGCAACCACTGGGTGGTCAACCCTCGGCACAACCTGGGCTTACAGCTCTCTGTGG
AGACCCTGGATGGGCAGAGCATCAACCCCAAGTTGGCAGGCCTGATTGGACGGCATGGACCCCAGAACAA
GCAACCCTTCATGGTGGCCTTCTTCAAGGCCACGGAAGTCCATCTCCGTAGTATCCGGTCCACGGGGGGC
AAGCAGCGCAGCCAGAATCGCTCCAAGACGCCAAAGAACCAAGAGGCCCTGAGGATGGCCAGTGTGGCAG
AAAACAGCAGCAGTGACCAGAGGCAGGCCTGCAAGAAACATGAGCTGTACGTCAGCTTCCGAGACCTTGG
CTGGCAGGACTGGATCATTGCACCTGAAGGCTATGCTGCCTACTACTGTGAGGGAGAGTGCGCCTTCCCT
CTGAACTCCTACATGAACGCCACCAACCACGCCATCGTCCAGACACTGGTTCACTTCATCAACCCAGACA
CAGTACCCAAGCCCTGCTGTGCGC
nt 550 - nt 1413 of NM_007557.2
Notes:The Bmp7 probe used in this study by Bulchand et al, 2001 [PMID:11165475] is described as such: "Digoxigenin-labeled probes were generated using T3, T7 or SP6 RNA polymerases from plasmids containing fragments of the appropriate genes, as described previously (Tole [PMID:10625551] , Tole [PMID:9362459] ; and Grove [PMID:9584130] )". Tole et al [PMID:10625551] describe a Bmp7 probe as "(0.8kb) Furuta et al., 1997 [PMID:9187146] ". Furuta et al define it as "a 860 bp fragment of Bmp7 containing the region homologous to nucleotides 450-1314 of human Bmp7". The co-ordinates shown above with reference to NM_007557.2 correspond to the homologous region in mouse.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Bulchand S; Grove EA; Porter FD; Tole S (2001) [PMID:11165475] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00515-3] [ PMID:11165475] Bulchand S, Grove EA, Porter FD, Tole S 2001 LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem. Mech Dev (100):165-75
 [ doi:10.1006/dbio.1999.9509] [ PMID:10625551] Tole S, Ragsdale CW, Grove EA 2000 Dorsoventral patterning of the telencephalon is disrupted in the mouse mutant extra-toes(J). Dev Biol (217):254-65
 [ PMID:9362459] Tole S, Christian C, Grove EA 1997 Early specification and autonomous development of cortical fields in the mouse hippocampus. Development (124):4959-70
 [ PMID:9584130] Grove EA, Tole S, Limon J, Yip L, Ragsdale CW 1998 The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development (125):2315-25
 [ PMID:9187146] Furuta Y, Piston DW, Hogan BL 1997 Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development. Development (124):2203-12
Links:MGI:2386917 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI