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EMAGE:3604

Hand1 heart and neural crest derivatives expressed transcript 1 ( MGI:103577)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:3604 EMAGE:3604 EMAGE:3604 EMAGE:3604
Fig2H.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig2F.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig2G.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002 Fig2I.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00093-5] Mech Dev 119 Suppl 1: S65-71, Smart N; Hill AA; Cross JC; Riley PR, A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis. Copyright 2002

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Approximate location of these transverse sections are shown in Fig2F. Image annotations: avc, atrio-ventricular canal; ba, branchial arch; bc, bulbis cordis; cc, cardiac neural crest; e, endocardium; ec, endocardial cushion; la, left atrium; lpm, lateral plate mesoderm; lv, left ventricle; m, myocradium; ot, outflow tract; ra, right atrium. In Fig2I, the arrows indicate relative orientations of the anterior-posterior and right-left axes.
Expression Pattern Description
Spatial Annotation:
EMAGE:3604EMAGE:3604EMAGE:3604EMAGE:3604Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3604_wholemount_strong_3D_3.wlz
3604_wholemount_moderate_3D_1.wlz
3604_wholemount_notDetected_3D_3.wlz
3604_voxel_notDetected_3D_1.wlz
3604_voxel_strong_3D_1.wlz
3604_voxel_notDetected_3D_2.wlz
3604_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3604_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
outflow tract
detected detected
regionalsee Fig2F, 2G, 2H, 2I. Expression is in the myocardium of the outflow tract and in the truncus arteriosus of the proximal outflow tract.
primitive ventricle
detected detected
see Fig2F, 2G, 2H, 2I.
trunk mesenchyme
detected detected
regionalExpression is in the lateral plate mesoderm (see Fig 2G)
1st branchial arch
detected detected
regionalExpression is in the medial region (see Fig2G). Expression is in the presumptive cardiac neural crest migrating through the pharyngeal arch region (see Fig2H).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:25809
Entity Detected:Hand1, heart and neural crest derivatives expressed transcript 1 ( MGI:103577)
Notes:Tha Hand1 (Hxt) probe used in this study by Smart et al, 2002 [PMID:14516662] is indicated as that used by Cross et al, 1995 [PMID:7671815] i.e. "RNA probes were made by preparing transcripts", "from the 3' end of mouse Hxt cDNA that had been cloned into pBluescript". Editors note: probe sequence start and end points are not defined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Smart N; Hill AA; Cross JC; Riley PR (2002) [PMID:14516662] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(03)00093-5] [ PMID:14516662] Smart N, Hill AA, Cross JC, Riley PR. 2002 A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis Mech Dev (119 Suppl 1):S65-71
 [ PMID:7671815] Cross JC, Flannery ML, Blanar MA, Steingrimsson E, Jenkins NA, Copeland NG, Rutter WJ, Werb Z 1995 Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development. Development (121):2513-23
Links:MGI:2671316 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI