EMAGE mouse gene expression spatial database

EMAGE:3613

Tle3 transducin-like enhancer of split 3, homolog of Drosophila E(spl) ( MGI:104634)
TS11 (7.5 dpc)
in situ hybridisation

Data Images


Fig2B.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/0925-4773(96)00582-5] Mech Dev 59: 73-87, Koop KE; MacDonald LM; Lobe CG, Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development. Copyright 1996

Expression pattern clarity:
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Notes:

Note: Arrow indicates the lateral plate mesoderm. Arrowhead indicates the node. Embryo oriented with anterior towards the left.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo mesoderm

detected

regional

Expression is in lateral mesoderm.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3044200
Entity Detected:	Tle3, transducin-like enhancer of split 3, homolog of Drosophila E(spl) ( MGI:104634)
Sequence:	sense strand is shown >MGI:3044200 AATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTATGACAAGCTGGCTAACGAGAAGAC GGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGCTTGAATATTGAAATGCACAAGCAG ACAGAGATTGCGAAGAGACTGAACACAATCCTAGCCCAGATCATGCCTTTTTTGTCACAGGAGCATCAGC AGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGACGGAGTTGAACGCCATCATCGGGCA GCAGCAACTCCAGGCCCAGCACCTCTCCCATGCCACGCATGGTCCCCCGGTCCAGCTGCCACCCCACCCG TCAGGCCTCCAGCCTCCTGGGATTCCCCCAGTGACAGGAAGCAGCTCTGGGTTGCTGGCACTTGGTGCCC TGGGAAGTCAAGCTCACTTGGCGGTGAAGGATGAGAAGAACCACCATGAACTGGATCACAGAGAGAGAGA GTCCAGCACGAACAATTCCGTGTCACCCTCTGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCTGCA GACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAAGAGAAAGACAGCCTCAGCAGATACGATAGCGATG GGGACAAGAGTGACGA
	nt 350 - nt 995 of NM_009389.1
Notes:	The Tle3 (Grg3) probe used in this study by Koop et al., 1996 [PMID:8892234] is defined as a "fragment covering the Q domain (Leon & Lobe., 1996 [PMID:8989517] )". Leon & Lobe define this probe as "350 to 995 bp in Fig. 2A, which would recognize both Grg3a and Grg3b". Editors Note: According to Fig 2A in Leon & Lobe, the probe sequence starts at an EcoRI site which is also found at position 350 in the Tle3 cDNA RefSeq NM_009389.1, however the sequence published in Fig2A is only 900nt long which has prevented cross-checking of the 3'end.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Koop KE; MacDonald LM; Lobe CG (1996) [PMID:8892234] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0925-4773(96)00582-5] [ PMID:8892234] Koop KE, MacDonald LM, Lobe CG 1996 Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development. Mech Dev (59):73-87
	[ doi:10.1002/(SICI)1097-0177(199701)208:1<11::AID-AJA2>3.0.CO;2-4] [ PMID:8989517] Leon C, Lobe CG 1997 Grg3, a murine Groucho-related gene, is expressed in the developing nervous system and in mesenchyme-induced epithelial structures. Dev Dyn (208):11-24
Links:	MGI:3044353 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source