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EMAGE:3618

Neurod1 neurogenic differentiation 1 ( MGI:1339708)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3618 EMAGE:3618
Figure 7-NeuroD. Copyright: This image is from Daubas P, Development 2000;127():319-331, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 7-NeuroD. Copyright: This image is from Daubas P, Development 2000;127():319-331, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: one star
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Neurod1 transcripts are detected by the blue strain. Image annotations: mv, mesencephalic vesicle. Arrows indicate Myf5-nlacZ expressing areas in the mantle layer of the neuroepithelium (red). Right panel is enlargement of panel shown on the left.
Expression Pattern Description
Spatial Annotation:
EMAGE:3618EMAGE:3618Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3618_voxel_strong_3D_1.wlz
3618_voxel_moderate_3D_1.wlz
3618_voxel_possible_3D_1.wlz
3618_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3618_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalExpression is detected in the mantle layer cells of the mesencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1351282
Entity Detected:Neurod1, neurogenic differentiation 1 ( MGI:1339708)
Notes:The Neurod1 probe used in this study by Daubas et al., 2000 [PMID:10603349] was "a 700 bp EcoRI-KpnI fragment of mouse coding sequence, kindly provided by F. Guillemot)". Editors Note: Restriction digestion of the mouse Neurod1 RefSeq NM 010894.2 with EcoRI and KpnI fails to identify a 700bp fragment. Probe region covered cannot be sepcified using information provided.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Myf5+/Myf5tm1Pas
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Daubas P; Tajbakhsh S; Hadchouel J; Primig M; Buckingham M, 2000 [PMID:10603349] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10603349] Daubas P, Tajbakhsh S, Hadchouel J, Primig M, Buckingham M 2000 Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development (127):319-31
Links:MGI:1352726 same experiment
 MGI:1857973 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI