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EMAGE:3664

Foxd3 forkhead box D3 ( MGI:1347473)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:3664 EMAGE:3664
Figure 2A. Copyright: This image is from Dottori et al., Development 128:4127-4138 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 2B. Copyright: This image is from Dottori et al., Development 128:4127-4138 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrows, migrating cranial neural crest cells; arrowhead, premigratory crest; sc, spinal cord. 2B is a section through the same stage embryo as shown in 2A.
Expression Pattern Description
Spatial Annotation:
EMAGE:3664Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3664_wholemount_strong_3D_1.wlz
3664_wholemount_moderate_3D_1.wlz
3664_wholemount_possible_3D_1.wlz
3664_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3664_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme derived from neural crest
detected detected
Expression neural crest cells emigrating from the dorsal neural tube along its entire length from midbrain/hindbrain border to the tail.
trunk mesenchyme derived from neural crest
detected detected
Expression neural crest cells emigrating from the dorsal neural tube along its entire length from midbrain/hindbrain border to the tail.
tail mesenchyme derived from neural crest
detected detected
Expression neural crest cells emigrating from the dorsal neural tube along its entire length from midbrain/hindbrain border to the tail.
neural tube
detected detected
regionalExpression in premigratory crest cells.
tail neural crest
detected detected
regionalExpression in premigratory crest cells.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1327558
Entity Detected:Foxd3, forkhead box D3 ( MGI:1347473)
Sequence:sense strand is shown

>MGI:1327558
GGATCCGGGCGCCCGGGGGCTCGGCTGGGGGTGGTGGGAGCGGCGGCGGAGCCGGGGGTGGCGGGGGCAG
TGGGGGCGGCGGCGGCGCGCAGTCCTTCCTGCGGCCGCCCGGGACTGTGCAGTCGGCTGCGCTCATGGCC
ACGCACCAGCCCCTGTCGCTGAGCCGGACGACTGCCACCATCGCGCCCATTCTGAGCGTGCCGCTGTCTG
GACAGTTCCTGCAGCCCGCAGCCTCAGCCGCAGCGGCTGCGGCCGCCGCCGTGCAAGCCAAGTGGCCCGC
TCAATAGTGATGAGCTAGTGGCCGGGCCGGACGGCGGCCTCTGGCAGCAGCCTGCTCTTCCAGGCGGTAC
GCCCTCTCTGATCCTGGTCCATCTGTCCTGGTCCTGGTCCTGGTCCCTCTACCCAATCCTGGACTCTGCT
ACCCGCCCCCGACTCCCCAACACTGACCAACAGCGGCCTCCATCCCCTCACTCACCTAAGCTGGCGGAGC
AAACTCAACCCGTCCGCTGGGAATAACTTTCCAGTACAGGTAAAACTGATAACCGAATTTCCAAAAATGC
ACCCCGACGGCGCCGTTGTTCTCAGTACTGTGGGGGTGGGAGGGACATTCTTTGTACATATTTGTATAAA
AATCATTGACTTTCCTTTTGGGGTTTTTATTTTTTTAAGAAAATAAAAAGCAATTCAGTAGATTTAGAGC
TTTGAACTTTCATATTTTTTTGAAGGTTCACTCTGCGCAGTTTTATCTGAGAAAAGAATGTATAAAGACT
TGGGAGATTTAAATACAAAAAATTTTTCAAAAAGGCGAAGTGTCATTGTATTATAAAAGTCTGTTTATAT
ATGAATGAATATATATGGTATTCTAAATGAGCGGC
nt 1431 - nt 2305 of AF067421.1
Notes:The Foxd3 (Hfh2) probe used in this study by Dottori et al., 2001 [PMID:11684651] was originally described in Labosky & Kaestner, 1998 [PMID:9767163] as "a 874 bp fragment of Hfh2 (positions 1431 to 2305 of the cDNA) which excludes the WH [winged helix] DNA binding motif". Editors Note: The cDNA referred to above was cloned by Labosky & Kaestner and deposited in Genbank under AF067421.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Dottori M; Gross MK; Labosky P; Goulding M, 2001 [PMID:11684651] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11684651] Dottori M, Gross MK, Labosky P, Goulding M 2001 The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. Development (128):4127-38
 [ doi:10.1016/S0925-4773(98)00105-1] [ PMID:9767163] Labosky PA, Kaestner KH 1998 The winged helix transcription factor Hfh2 is expressed in neural crest and spinal cord during mouse development. Mech Dev (76):185-90
Links:MGI:2152678 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI