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EMAGE:3668

Foxa2 forkhead box A2 ( MGI:1347476)
TS10 (MS Downs & Davies)
immunohistochemistry

Data Images
EMAGE:3668
Figure 1B. Copyright: This image is from Filosa et al., Development 124: 2843-2854 (1997), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
HNF-3beta [FOXA2] protein staining is brown and Gsc RNA staining is purple. *** only the FOXA2 protein expression domain is described and annotated in this EMAGE entry*** Image annotations: Arrows show cells that express both Gsc and Hnf3b, while arrowheads point out regions that express only Hnf3b. Scale bar, 100 microns.
Expression Pattern Description
Spatial Annotation:
EMAGE:3668EMAGE:3668Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3668_voxel_strong_3D_1.wlz
3668_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3668_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive streak
detected detected
Expression is detected in all three germ layers at the anterior end of the primitive streak. Expression domain encompasses the domain of expression of the Gsc gene in the anterior primitive streak.
primitive endoderm
detected detected
expression in visceral endoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1338477
Entity Detected:Foxa2, forkhead box A2 ( MGI:1347476)
Notes:The anti-FOXA2 antisera used in this study by Filosa et al., 1997 [PMID:9226455] was described as "anti-HNF-3b antibodies at a dilution of 1:500".
Antibody Type:polyclonal
Specimen
Organism:mouse
Strain:involves: 129/Sv * C57BL/6 * CD-1
Age:MS Downs & Davies
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:7.5% gelatin
General Information
Authors:Filosa S; RiveraPerez JA; Gomez AP; Gansmuller A; Sasaki H; Behringer RR; Ang SL, 1997 [PMID:9226455] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9226455] Filosa S, Rivera-Perez JA, Gomez AP, Gansmuller A, Sasaki H, Behringer RR, Ang SL 1997 Goosecoid and HNF-3beta genetically interact to regulate neural tube patterning during mouse embryogenesis. Development (124):2843-54
Links:MGI:1338493 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI