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EMAGE:3670

Foxa2 forkhead box A2 ( MGI:1347476)
TS09 (6.5 dpc)
immunohistochemistry

Data Images
EMAGE:3670 EMAGE:3670
Figure 1H. Copyright: This image is from Perea-Gomez et al., Development 126: 4499-4511 (1999), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 1K. Copyright: This image is from Perea-Gomez et al., Development 126: 4499-4511 (1999), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
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Notes:
Note: (H) Whole-mount in situ hybridisation using Lhx1 (Lim1) RNA probe (purple) and immunohistochemistry with anti-HNF3beta [FOXA2] antibody (brown). The level of the transverse section depicted in K is indicated by the horizontal black line in H. Arrowhead in H, HNF3beta [FOXA2] expression in most anterior cells of the primitive streak. Arrow in K, anterior visceral endoderm; arrowhead in K, region more posterior of co-expression of Hnf3beta and Lhx1; short arrow in K, Lhx1 expression in anterior mesoderm wings. Anterior is to the left in H, and to the top in K. Abbreviations: a, anterior; p, posterior. Scale bar, 50 microns.
Expression Pattern Description
Spatial Annotation:
EMAGE:3670EMAGE:3670Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingwholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3670_wholemount_strong_3D_2.wlz
3670_wholemount_moderate_3D_2.wlz
3670_wholemount_possible_3D_1.wlz
3670_wholemount_notDetected_3D_2.wlz
3670_voxel_strong_3D_1.wlz
3670_voxel_moderate_3D_1.wlz
3670_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3670_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive streak
detected detected
regional(H) HNF3beta [FOXA2] expression domain extends more distal than the Lhx1 (Lim1) domain. (K) HNF3beta and Lhx1 (Lim1) are coexpressed in the anterior primitive streak.
primitive endoderm
detected detected
HNF3beta [FOXA2] and Lhx1 (Lim1) are coexpressed in the anterior visceral endoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1338477
Entity Detected:Foxa2, forkhead box A2 ( MGI:1347476)
Notes:The anti-FOXA2(HNF3-beta) antisera used in this study by Perea-Gomez et al, 1999 [PMID:10498685] is described as that used by Filosa et al., 1997 [PMID:9226455] i.e. "anti-HNF-3b antibodies at a dilution of 1:500".
Antibody Type:polyclonal
Specimen
Organism:mouse
Age:6.5 dpc
Theiler Stage:TS09
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
General Information
Authors:Perea-Gomez A; Shawlot W; Sasaki H; Behringer RR; Ang S, 1999 [PMID:10498685] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10498685] Perea-Gomez A, Shawlot W, Sasaki H, Behringer RR, Ang S 1999 HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo. Development (1999):4499-511
 [ PMID:9226455] Filosa S, Rivera-Perez JA, Gomez AP, Gansmuller A, Sasaki H, Behringer RR, Ang SL 1997 Goosecoid and HNF-3beta genetically interact to regulate neural tube patterning during mouse embryogenesis. Development (124):2843-54
Links:MGI:1346356 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI