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EMAGE:3678

Necab2 N-terminal EF-hand calcium binding protein 2 ( MGI:2152211)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3678 EMAGE:3678
Figure 3A. Copyright: This image is from Bernier et al., Development 128:3987-3994 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 3C. Copyright: This image is from Bernier et al., Development 128:3987-3994 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: fb, forebrain; nr, neural retina; op, the olfactory placodes; poa, pre-optic area; rpe, retinal pigmented epithelium. A, arrows, expresseion in the dorsal portion of the midbrain.
Expression Pattern Description
Spatial Annotation:
EMAGE:3678EMAGE:3678EMAGE:3678Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3678_voxel_possible_3D_1.wlz
3678_voxel_notDetected_3D_1.wlz
3678_voxel_strong_3D_1.wlz
3678_voxel_possible_3D_2.wlz
3678_voxel_notDetected_3D_2.wlz
3678_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3678_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
optic cup inner layer
detected detected
Fig 3A.
optic cup outer layer
detected detected
Fig3A.
midbrain
detected detected
regionalExpression in the dorsal portion of the midbrain (Fig3A)
forebrain
detected detected
Fig 3C
nose
detected detected
Expression in the olfactory placodes. (Fig 3C). Expression is the same as Pax6 (see MGI:2153747).
eye
detected detected
Expression in the pre-optic area (Fig 3C).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2152925
Entity Detected:Necab2, N-terminal EF-hand calcium binding protein 2 ( MGI:2152211)
Notes:The Efcbp2 (Necab) probe used in this study by Bernier et al., 2001 [PMID:11641222] is described as a "1.9 kb Necab [Efcbp2] cDNA (clone 9066)". Editors note: The authors also state elsewhere in this paper: "Using a previously described large-scale in situ screen for genes expressed in the developing mouse embryo (Neidhardt et al., 2000 [PMID:11044609] ), a novel cDNA of 1.9 kb (clone 9066) showing expression in the optic vesicle was isolated. This cDNA fragment was used to screen a 15.5 dpc mouse embryo cDNA library. Three independent clones were isolated, sequenced and compared to the clone 9066. It was found that clone 9066 had a 200 bp non-coding insertion between exon 1 and 2."
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Bernier G; Vukovich W; Neidhardt L; Herrmann BG; Gruss P, 2001 [PMID:11641222] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11641222] Bernier G, Vukovich W, Neidhardt L, Herrmann BG, Gruss P 2001 Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development (128):3987-94
 [ doi:10.1016/S0925-4773(00)00453-6] [ PMID:11044609] Neidhardt L, Gasca S, Wertz K, Obermayr F, Worpenberg S, Lehrach H, Herrmann BG 2000 Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos. Mech Dev (98):77-94
Links:MGI:2153746 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI