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EMAGE:3680

Necab2 N-terminal EF-hand calcium binding protein 2 ( MGI:2152211)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:3680
Figure 2E. Copyright: This image is from Bernier et al., Development 128:3987-3994 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Black arrowhead points to optic vesicle. White arrowheads point to the pretectum.
Expression Pattern Description
Spatial Annotation:
EMAGE:3680Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3680_wholemount_strong_3D_1.wlz
3680_wholemount_moderate_3D_1.wlz
3680_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3680_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
prosencephalon
detected detected
optic vesicle
detected detected
Expression resembled that of Pax6 (MGI:2153750).
future midbrain roof plate
detected detected
Expression in the pre-tectum. Expression resembled that of Pax6 (MGI:2153750).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2152925
Entity Detected:Necab2, N-terminal EF-hand calcium binding protein 2 ( MGI:2152211)
Notes:The Efcbp2 (Necab) probe used in this study by Bernier et al., 2001 [PMID:11641222] is described as a "1.9 kb Necab [Efcbp2] cDNA (clone 9066)". Editors note: The authors also state elsewhere in this paper: "Using a previously described large-scale in situ screen for genes expressed in the developing mouse embryo (Neidhardt et al., 2000 [PMID:11044609] ), a novel cDNA of 1.9 kb (clone 9066) showing expression in the optic vesicle was isolated. This cDNA fragment was used to screen a 15.5 dpc mouse embryo cDNA library. Three independent clones were isolated, sequenced and compared to the clone 9066. It was found that clone 9066 had a 200 bp non-coding insertion between exon 1 and 2."
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:autoradiography
General Information
Authors:Bernier G; Vukovich W; Neidhardt L; Herrmann BG; Gruss P, 2001 [PMID:11641222] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11641222] Bernier G, Vukovich W, Neidhardt L, Herrmann BG, Gruss P 2001 Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development (128):3987-94
 [ doi:10.1016/S0925-4773(00)00453-6] [ PMID:11044609] Neidhardt L, Gasca S, Wertz K, Obermayr F, Worpenberg S, Lehrach H, Herrmann BG 2000 Large-scale screen for genes controlling mammalian embryogenesis, using high-throughput gene expression analysis in mouse embryos. Mech Dev (98):77-94
Links:MGI:2153749 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI