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EMAGE:3687

Adamts20 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20 ( MGI:2660628)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3687
Figure 5B. Copyright: This image is from Rao et al., Development 130, 4665-4672 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3687Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3687_wholemount_strong_3D_1.wlz
3687_wholemount_moderate_3D_1.wlz
3687_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3687_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3530546
Entity Detected:Adamts20, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20 ( MGI:2660628)
Notes:The Adamts20 (Adex3/8) probe used in this study by Rao et al., 2003 [PMID:12925592] was described as follows: "Reverse transcribed digoxigenin-conjugated probes was made from linearized plasmid Adex3/8". Elsewhere in the paper the authors describe that the insert of plasmid Adex3/8 was generated by PCR using Adex3/8F, 5'-GGCTTAGCAGAGCTAGGCACCC-3'; and Adex3/8R, 5'-GTTGTCACCACCACACAC-3'. Editor note: Pairwise BLAST between Adex3/8R and the AdamTS20 RefSeq NM_177431.3 does not find a match whereas a match is found with Adex3/8F.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/NJ
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Rao C; Foernzler D; Loftus SK; Liu S; McPherson JD; Jungers KA; Apte SS; Pavan WJ; Beier DR, 2003 [PMID:12925592] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00668] [ PMID:12925592] Rao C, Foernzler D, Loftus SK, Liu S, McPherson JD, Jungers KA, Apte SS, Pavan WJ, Beier DR 2003 A defect in a novel ADAMTS family member is the cause of the belted white-spotting mutation. Development (130):4665-72
Links:MGI:3530570 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI