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EMAGE:3692

Tcf15 transcription factor 15 ( MGI:104664)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3692
Figure 2A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00323-3] Mech Dev 95: 35-46, Beckers J; Schlautmann N; Gossler A, The mouse rib-vertebrae mutation disrupts anterior-posterior somite patterning and genetically interacts with a delta1 null allele Copyright 2000.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: This embryo was stained for 4 hrs.
Expression Pattern Description
Spatial Annotation:
EMAGE:3692Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3692_wholemount_strong_3D_1.wlz
3692_wholemount_moderate_3D_1.wlz
3692_wholemount_weak_3D_1.wlz
3692_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3692_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk paraxial mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334537
Entity Detected:Tcf15, transcription factor 15 ( MGI:104664)
Notes:The Tcf15 (paraxis) probe used in this study by Beckers et al., 2000 [PMID:10906448] is indicated as that described by Burgess et al., 1995 [PMID:7729571] i.e. "synthesized from a 1.2kb paraxis cDNA insert cloned into pBSKII+". Editor Note: Burgess et al point out that the under the hybridisation conditions they used there is no cross-hybridisation of this probe to scleraxis.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Beckers J; Schlautmann N; Gossler A, 2000 [PMID:10906448] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00323-3] [ PMID:10906448] Beckers J, Schlautmann N, Gossler A 2000 The mouse rib-vertebrae mutation disrupts anterior-posterior somite patterning and genetically interacts with a Delta1 null allele. Mech Dev (95):35-46
 [ doi:10.1006/dbio.1995.1081] [ PMID:7729571] Burgess R, Cserjesi P, Ligon KL, Olson EN 1995 Paraxis: a basic helix-loop-helix protein expressed in paraxial mesoderm and developing somites. Dev Biol (168):296-306
Links:MGI:2384646 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI