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EMAGE:3702

Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A ( MGI:107558)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3702
Figure 8A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/0925-4773(96)00525-4] Mech Dev 57: 33-45, Adams RH; Betz H; Puschel AW, A novel class of murine semaphorins with homology to thrombospondin is differentially expressed during early embryogenesis. Copyright 1996.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Lateral view. Anterior is to the left and dorsal to the bottom. Arrow indicates posterior of somite. l, limb bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:3702Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3702_wholemount_strong_3D_1.wlz
3702_wholemount_moderate_3D_1.wlz
3702_wholemount_possible_3D_1.wlz
3702_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3702_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
homogeneous
trunk somite
detected detected
regionalExpression is posterior.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2149213
Entity Detected:Sema3a, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A ( MGI:107558)
Notes:The Sema3a (semD) probe used in this study by Adams et al., 1996 [PMID:8817451] is described as "a 2.9 kb cDNA fragment for semD (including codons for amino acids 275-772 and additional 3 -non-translated sequences)". Editor note: the probe 3' end point is undefined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Adams RH; Betz H; Puschel AW, 1996 [PMID:8817451] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/0925-4773(96)00525-4] [ PMID:8817451] Adams RH, Betz H, Puschel AW 1996 A novel class of murine semaphorins with homology to thrombospondin is differentially expressed during early embryogenesis. Mech Dev (57):33-45
Links:MGI:3044159 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI