EMAGE mouse gene expression spatial database

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EMAGE:3705

Rhob ras homolog gene family, member B ( MGI:107949)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.	Figure 2A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.	Figure 2B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.	Figure 2C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.	Figure 2C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.

Figure 2E. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00333-6] Mech Dev 95: 211-4, Henderson DJ; Ybot-Gonzalez P; Copp AJ, RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo Copyright 2000.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:
Find spatially similar expression patterns:

Notes:

Note: Dotted lines indicate level of sections. Arrows in a) point to paraxial mesoderm along the length of the embryo, arrowheads to branchial arches. b) section of the trunk region. R is rostral, C is caudal. Somite boundaries are marked with arrows. c) transverse section through the cranial region. d) section at the level of branchial arches, arrows point to branchial pouches. e) level of aortic sac, arrowheads point to branchial arch arteries as they join the aortic sac. Arrows point to developing motor columns in the ventral part of the neural tube (see also d, and f). f) arrowheads point to developing sensory axons extending fom the ventral aspect of the dorsal root ganglia. Arrow points to developing sympathetic chain adjacent to the paired dorsal aortae. a, dorsal aorta; as, aortic sac; drg, dorsal root ganglia; fg, foregut; nt, neural tube; sv, septum tranversum; 1-3, branchial arches 1,2 and 3; V, trigeminal ganglion; VII, facio-acoustic ganglion; IX, glosso-pharyngeal ganglion. Scale bar in a) 1.1mm; (b-f) 175 microns. Section B was not spatially annotated in this EMAGE entry due to a lack of features which has prevented spatial data placement.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

wholemount mapping

View mapped 3D expression image

Download individual expression domains:

3705_wholemount_strong_3D_4.wlz

3705_wholemount_moderate_3D_5.wlz

3705_wholemount_weak_3D_5.wlz

3705_wholemount_possible_3D_1.wlz

3705_wholemount_notDetected_3D_5.wlz

3705_voxel_moderate_3D_1.wlz

3705_voxel_weak_3D_1.wlz

3705_voxel_notDetected_3D_1.wlz

3705_voxel_moderate_3D_2.wlz

3705_voxel_weak_3D_2.wlz

3705_voxel_notDetected_3D_2.wlz

3705_voxel_strong_3D_1.wlz

3705_voxel_moderate_3D_3.wlz

3705_voxel_weak_3D_3.wlz

3705_voxel_notDetected_3D_3.wlz

3705_voxel_strong_3D_2.wlz

3705_voxel_moderate_3D_4.wlz

3705_voxel_weak_3D_4.wlz

3705_voxel_notDetected_3D_4.wlz

3705_voxel_strong_3D_3.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3705_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk paraxial mesenchyme

detected

branchial arch

detected

regional

Expression in streams of cells.

neural tube

detected

regional

Expressing cells migrating out of the dorsal part of the neural tube.

trigeminal v ganglion

detected

facial vii ganglion

detected

glossopharyngeal ix ganglion

detected

1st branchial pouch

detected

regional

Epithelium of pouches.

2nd branchial pouch

detected

regional

Epithelium of pouches.

3rd branchial pouch

detected

regional

Epithelium of pouches.

4th branchial pouch

detected

regional

Epithelium of pouches.

branchial arch artery

detected

outflow tract

detected

regional

Expression in aortic sac.

neural tube floor plate

detected

regional

Expression in the developing motor columns.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1862033
Entity Detected:	Rhob, ras homolog gene family, member B ( MGI:107949)
Sequence:	sense strand is shown >MGI:1862033 CGTGTTCGAGAACTATGTGGCGGACATCGAGGTGGACGGCAAGCAGGTGGAGCTGGCGCTGTGGGACACG GCAGGCCAGGAGGACTACGATCGTTTACGGCCGCTCTCCTATCCGGACACCGACGTCATCCTTATGTGCT TCTCGGTGGACAGCCCGGACTCTCTCGAGAACATCCCCGAGAAGTGGGTGCCCGAGGTAAAGCACTTCTG CCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGACCTGCGCAGCGACGAGCATGTCCGCACGGAG CTGGCCCGCATGAAGCAGGAGCCAGTGCGCACGGATGACGGCCGCGCCATGGCGGTGCGCATCCAAGCCT ATGACTACCTCGAGTGCTCGGCCAAGACCAAGGAGGGCGTGCGCGAGGTTTTTGAGACGGCCACGCGCGC CGCGCTGCAGAAGCGCTACGGATCCCAGAATGGCTGCATCAACT
	nt 4063 - nt 4526 of X99963.1
Notes:	The Rhob probe used in this study by Henderson et al., 2000 [PMID:10906464] "was obtained from GenBank [sequence] (accession number x99963) and a 463 bp fragment was amplified by RT-PCR. This corresponds to the region between positions 4063 and 4526 of the cDNA sequence".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Henderson DJ; Ybot-Gonzalez P; Copp AJ, 2000 [PMID:10906464] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(00)00333-6] [ PMID:10906464] Henderson DJ, Ybot-Gonzalez P, Copp AJ 2000 RhoB is expressed in migrating neural crest and endocardial cushions of the developing mouse embryo. Mech Dev (95):211-4
Links:	MGI:2384277 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source